CORE--TISSUE CULTURE AND MHC TYPING CORE

Core B是组织培养和MHC分型Core。 在这个机构里，我们 执行所有样品处理，包括以下各项： 外周血淋巴细胞，冻存，解冻和洗涤 冷冻细胞和B细胞系的制备。 核心还执行 以下检测：所有新样本的HLA-A、B、C血清学分型 作为HLA-DR，DQ血清学在一些选定的样品。 另外核心 是否使用序列特异性DNA分型HLA II类等位基因 寡核苷酸探针杂交（SSOPH）和DNA分型HLA-C使用 序列特异性引物（PCR-SSP）。 其他类型，如补码 蛋白质分析也在新样品上进行。

Core B is a tissue culture and MHC typing Core. In this facility, we perform all sample processing that includes the following; isolation of peripheral blood lymphocytes, cryopreservation, thawing and washing of frozen cells and preparation of B cell lines. The Core also perform the following tests: HLA-A, B, C typing by serology on all new samples as well as HLA-DR, DQ serology on a few selected samples. In addition, the Core does DNA typing for HLA Class II alleles using sequence specific oligonucleotide probe hybridization (SSOPH) and DNA typing for HLA-C using sequence-specific primers (PCR-SSP). Other typings, such as complement protein analysis, are also performed on new samples.