Development and application of eDNA tools to assess the structure and function of coastal sea ecosystems (MARINe-DNA)

评估沿海海洋生态系统结构和功能的eDNA工具的开发和应用（MARINe-DNA）

• 批准号: NE/N006100/1
• 负责人: William Wilson
• 金额: $ 38.1万
• 依托单位: Marine Biological Association of the United Kingdom
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FN006100%2F1
• 关键词: Development; application; eDNA; tools; assess

This NERC highlight topic focuses on the use of eDNA as a new tool for 21st century ecology. Environmental DNA (eDNA) is defined in the call as 'free DNA present outside of any organism'. The aim of the call is to address the current knowledge gaps in the application of eDNA approaches to help understand community biodiversity and dynamics of ecosystem functioning. We will conduct a proof-of-concept investigation at Station L4, an exemplar coastal ocean ecosystem, and natural laboratory, in the English Channel off Plymouth, UK. Starting with a hydrodynamic model to spatially and temporally define the ecosystem (how large is the natural laboratory?) the project will then be split into three experimental phases: 1) eDNA methodological validation (developing the tools);2) 18-month temporal pelagic survey (testing the tools); and3) Comprehensive data analysis and model assimilation (did the tools work, what did they tell us, and are they useful?)Using a wide range of expertise from 4 different institutions (PML, MBA, NOC, and U.Exeter), we will investigate a spatially defined region, from estuarine to coastal, benthic to pelagic; and at a range of temporal resolutions building on NERC National Capability sampling regimes and biosensor deployment. E-metagenetic and e-metagenomic data (individual genes to whole genomes) will be used to answer cross-cutting science questions utilising current physicochemical and biological information collected in parallel at this important coastal site. Results from this project will provide a methodological template for the use of eDNA and remote eDNA biosensors in aquatic ecosystems. Downstream data will significantly advance our understanding of persistence of eDNA, and its potential impact on informing models of ecosystem functioning. Products of this research will have wider implications for the use of this tool on fisheries assessments, fish pathogen detection, conservation biology, environmental risk management (e.g. toxic algae blooms, human pathogens, ballast water regulations), with the wider aim of supporting biodiversity and nature's services through NERC's strategic pillar of "Managing environmental change".

这个NERC突出主题的重点是使用eDNA作为一个新的工具，为21世纪的世纪生态。环境DNA（eDNA）是“存在于任何生物体外部的游离DNA”。该呼吁的目的是解决目前在应用eDNA方法方面的知识差距，以帮助了解社区生物多样性和生态系统功能的动态。我们将在L4站进行概念验证调查，这是一个典型的沿海海洋生态系统和自然实验室，位于英国普利茅斯附近的英吉利海峡。从流体动力学模型开始，在空间和时间上定义生态系统（天然实验室有多大？）然后，该项目将分为三个实验阶段：1）eDNA方法学验证（开发工具）;2）为期18个月的时间远洋调查（测试工具）; 3）综合数据分析和模式同化（工具是否有效，它们告诉我们什么，它们有用吗？）我们将利用来自4个不同机构（PML、MBA、NOC和U.埃克塞特）的广泛专业知识，调查空间定义的区域，从河口到沿海，从海底到远洋;并以一系列时间分辨率建立在NERC国家能力采样制度和生物传感器部署的基础上。E-metagenetic和e-metagenomic数据（单个基因到整个基因组）将用于回答交叉科学问题，利用在这个重要的沿海站点并行收集的当前物理化学和生物学信息。该项目的结果将为在水生生态系统中使用eDNA和远程eDNA生物传感器提供方法模板。下游数据将大大推进我们对eDNA持久性的理解，以及它对生态系统功能模型的潜在影响。这项研究的产品将对渔业评估，鱼类病原体检测，保护生物学，环境风险管理（例如有毒藻类水华，人类病原体，压载水法规）的工具的使用产生更广泛的影响，更广泛的目标是通过NERC的战略支柱“管理环境变化”来支持生物多样性和自然服务。

项目成果

Influence of short and long term processes on SAR11 communities in open ocean and coastal systems.

• DOI: 10.1038/s43705-022-00198-1
• 发表时间: 2022-11-19
• 期刊: ISME COMMUNICATIONS
• 作者: Bolanos, Luis M.;Tait, Karen;Somerfield, Paul J.;Parsons, Rachel J.;Giovannoni, Stephen J.;Smyth, Timothy;Temperton, Ben
• 通讯作者: Temperton, Ben

Cryptic bacterial pathogens of diatoms peak during senescence of a winter diatom bloom.

硅藻的隐性细菌病原体在冬季硅藻花的衰老过程中达到顶峰。

• DOI: 10.1111/nph.19441
• 发表时间: 2023
• 期刊: The New phytologist
• 作者: Branscombe L

Sequence locally, think globally: The Darwin Tree of Life Project.

本地排序，全球思考：达尔文生命之树项目。

• DOI: 10.1073/pnas.2115642118
• 发表时间: 2022-01-25
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Darwin Tree of Life Project Consortium

The genome sequence of the grey top shell, Steromphala cineraria (Linnaeus, 1758)

灰顶壳 Steromphala cineraria 的基因组序列（Linnaeus，1758）

• DOI: 10.12688/wellcomeopenres.17677.1
• 发表时间: 2022
• 期刊: Wellcome Open Research
• 作者: Adkins P

Testing Bergmann's rule in marine copepods

• DOI: 10.1111/ecog.05545
• 发表时间: 2021-08-10
• 期刊: ECOGRAPHY
• 影响因子: 5.9
• 作者: Campbell, Max D.;Schoeman, David S.;Richardson, Anthony J.
• 通讯作者: Richardson, Anthony J.