LEICA TCS 4D UV VIS CONFOCAL MICROSCOPE

LEICA TCS 4D 紫外可见共焦显微镜

• 批准号: 2040546
• 负责人: WILLIAM A CATTERALL
• 金额: $ 37.83万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-15 至 2001-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2040546
• 关键词: biomedical equipment purchase; confocal scanning microscopy; digital imaging

This proposal requests support for an inverted confocal microscope with UV laser to provide technology which is currently unavailable to and is pivotal for the research effort of ten independent laboratories in the biomedical sciences. Research programs in all ten laboratories are now at a critical phase where long term access to this equipment is essential for further progress. Eight NIH funded users and two other funded groups are identified whose research programs include the following areas of investigation: Cell division, development, structure and function of opioid receptors, long term potentiation, cyclic nucleotide phosphodiesterases, synaptogenesis and synaptic structure, membrane traffic and redistribution of organelles within a cell and the associated changes in second messengers, interactions between calmodulin and neuromodulin, a major constituent of neuronal growth cones, and the expression and localization of voltage-gated ion chamois at the neuromuscular junction and throughout the brain. The broad range of projects proposed by the initial user group is representative of a larger group of potential users of confocal microscopy. The requested instrument would be placed in the established Keck Imaging Facility located on the University of Washington and be made available to other investigators when it is not being used by the core group of researchers. The operation and management of the equipment, the internal advisory committee and institutional support for the confocal microscope are described.

该建议要求支持倒置的共聚焦显微镜 使用紫外线激光器提供目前无法使用的技术 这对于十个独立的研究工作至关重要 生物医学的实验室。全部十个研究计划 实验室现在处于关键阶段，长期访问 该设备对于进一步进步至关重要。八项NIH资助 确定了用户和其他两个资助的小组的研究 计划包括以下调查领域：细胞部门， 阿片受体的开发，结构和功能，长期 增强，环状核苷酸磷酸二酯酶，突触发生 和突触结构，膜流量和重新分布 细胞内的细胞器以及第二个相关的变化 使者，钙调蛋白和神经模块蛋白之间的相互作用， 神经元生长锥的组成部分，表达和 电压门控离子木耳的定位在神经肌肉 交界处和整个大脑。广泛的项目 初始用户组提出的代表较大的 共聚焦显微镜的潜在用户组。请求 仪器将放置在既定的凯克成像设施中 位于华盛顿大学，可用于 其他调查人员在不使用核心组时 研究人员。设备的操作和管理， 内部咨询委员会和机构支持 描述了共聚焦显微镜。