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Leica SP8 WWL Confocal Microscope

Leica SP8 WWL 共焦显微镜

基本信息

批准号：
8639342
负责人：
WILLIAM A CATTERALL
金额：
$ 58.13万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-15 至 2015-06-14
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): We are requesting funds for the purchase of a Leica SP8 WLL confocal microscope. The instrument will be placed in the University of Washington's Keck Imaging Facility. The Keck Facility is a well- established, very heavily used facility providing confocal microscope instrumentation for 20 years, serving over 50 UW research laboratories in 2012. The facility now has two outdated and well-worn confocal microscopes, one of which is no longer supported by its manufacturer. The requested instrument is required to sustain microscope access for our group of 50 users and to provide expanded technical capabilities that are necessary to a group of 6 major users for advancing research projects ranging from dynamics within single cells to development of entire embryos. Thus, the proposed instrument will be required to analyze a vast diversity of specimens, in both fixed and living conditions. Significantly, the proposed instrument will make unique capabilities and badly needed additional confocal capacity available to the entire Keck user base and larger UW research community. It is to achieve these goals that we are requesting the Leica SP8 WLL confocal microscope with an inverted, DMI 6000 microscope stand. The system is specified with a white light laser (WLL) providing selection of any excitation wavelengths (up to 8 simultaneously) from 470 - 670 nm. Combined with Leica's extremely flexible spectral detection, fluorescent imaging parameters are user-customizable to a degree not currently available at the University of Washington. The system includes two hybrid (HyD) detectors for fluorescence. These new-generation detectors provide significantly increased sensitivity, wide dynamic range and low noise characteristics compared to standard detectors. A resonance scanner provides rapid imaging necessary for capturing dynamic events. A motorized stage and adaptive focus correction module are included for automated sampling and maintaining correct focus during time-lapse studies, respectively. A temperature-controlled stage insert is included for live-cell experiments. The Leica SP8 WLL was evaluated by the Keck Facility staff and major users at a recent demonstration and found to meet and exceed all of our expectations. In summary, the proposed instrument will permit users to optimize confocal imaging parameters, in ways currently impossible, to overcome the specimen's limitations rather than forcing experimental compromises necessary to overcome current instrument limitations.

描述(由申请人提供)：我们正在申请购买徕卡SP8WLL共焦显微镜的资金。该仪器将被放置在华盛顿大学的凯克成像设施中。凯克实验室是一家久负盛名、使用量很大的设施，提供20年的共焦显微镜仪器，2012年为50多个华盛顿大学研究实验室提供服务。该工厂现在有两台过时且磨损严重的共焦显微镜，其中一台不再受到制造商的支持。所要求的仪器需要为我们的50名用户提供显微镜访问，并为6名主要用户提供必要的扩展技术能力，以推进从单细胞内的动力学到整个胚胎开发的各种研究项目。因此，拟议的仪器将需要在固定和活着的条件下分析各种各样的标本。值得注意的是，建议的仪器将为整个Keck用户群和更大的UW研究社区提供独特的能力和迫切需要的额外共焦能力。正是为了实现这些目标，我们要求徕卡SP8 WLL共焦显微镜具有倒置的DMI6000显微镜支架。该系统采用白光激光器(WLL)，可选择470-670 nm范围内的任何激发波长(同时最高可达8个)。与徕卡极其灵活的光谱检测相结合，荧光成像参数可由用户定制，达到目前华盛顿大学所不具备的程度。该系统包括两个混合(HYD)荧光探测器。与标准探测器相比，这些新一代探测器具有更高的灵敏度、更宽的动态范围和更低的噪声特性。共振扫描仪提供捕捉动态事件所需的快速成像。包括电动平台和自适应焦点校正模块，分别用于在延时研究期间自动采样和保持正确的焦点。用于活细胞实验的温控舞台插入物包括在内。徕卡SP8 WLL在最近的一次演示中由凯克工厂的工作人员和主要用户进行了评估，发现它满足并超过了我们所有的预期。总之，拟议的仪器将允许用户以目前不可能的方式优化共焦成像参数，以克服样本的限制，而不是强迫进行必要的实验妥协，以克服当前仪器的限制。

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Photopatterned biomolecule immobilization to guide three-dimensional cell fate in natural protein-based hydrogels

DOI：
10.1073/pnas.2014194118
发表时间：
2021-01-26
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Batalov, Ivan;Stevens, Kelly R.;DeForest, Cole A.
通讯作者：
DeForest, Cole A.

Bioactive site-specifically modified proteins for 4D patterning of gel biomaterials