CORE--TISSUE CULTURE

组织培养研究核心支持囊性纤维化的SCOR 研究人员通过提供培养的人类上皮细胞和 动物处于与计划的实验相适应的条件下。这 总体目标通过四个具体目标来实现：1)组织 获取；2)患者特征；3)原代细胞培养；以及 4)细胞系培养。正常和囊性纤维化(CF患者计划 用于择期鼻部或肺部手术，其特征是 建立了临床和实验室标准。上皮细胞是 从过量切除的组织中分离并培养 保留必要的生理特性的技术 对拟议的研究的支持。专门化细胞纯化与培养 根据需要使用技术来实现特定的实验目标。 分离小鼠[正常或CFTR(-/-)]呼吸道上皮细胞 用类似的技术培养的。正常和CF上皮细胞系 表达了天然组织的基本特征的是 获得、培养并根据需要提供给SCOR调查人员以满足 总体实验目标。这些活动反映了集中化的 提供已成功支持CF的成熟技术 八年多的研究，以及较新试剂的适应 为这项任务。

The Tissue Culture Research Core supports SCOR in Cystic Fibrosis investigators by supplying cultured epithelial cells from humans and animals in conditions appropriate to the planned experiments. This overall goal is achieved through four Specific Aims: 1) tissue acquisition; 2) patient characterization; 3) primary cell culture; and 4) cell line culture. Normal and cystic fibrosis (CF patients scheduled for elective nasal or lung surgery are identified and characterized by established clinical and laboratory criteria. Epithelial cells are isolated from excess excised tissues and cultured by established techniques that preserve the physiological properties that are essential to the proposed studies. Specialized cell purification and culture techniques are used as needed to achieve specific experimental goals. Mouse [normal or CFTR(-/-)] airway epithelial cells are isolated and cultured by analogous techniques. Normal and CF epithelial cell lines that express the essential characteristics of the native tissue are obtained, cultured, and provided to SCOR investigators as needed to meet overall experimental goals. These activities reflect the centralized provision of established techniques that have successfully supported CF research for more than eight years, and the adaptation of newer reagents to this mission.