CORE--TISSUE CULTURE

核心——组织培养

• 批准号: 6109993
• 负责人: JAMES R YANKASKAS
• 金额: --
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6109993
• 关键词: athymic mouse; biomedical facility; cystic fibrosis; electrophysiology; human subject; human tissue; laboratory rat; patient /disease registry; postmortem; respiratory epithelium; tissue /cell culture; tissue resource /registry

The Tissue Culture Research Core supports SCOR in Cystic Fibrosis investigators by supplying cultured epithelial cells from humans and animals in conditions appropriate to the planned experiments. This overall goal is achieved through four Specific Aims: 1) tissue acquisition; 2) patient characterization; 3) primary cell culture; and 4) cell line culture. Normal and cystic fibrosis (CF patients scheduled for elective nasal or lung surgery are identified and characterized by established clinical and laboratory criteria. Epithelial cells are isolated from excess excised tissues and cultured by established techniques that preserve the physiological properties that are essential to the proposed studies. Specialized cell purification and culture techniques are used as needed to achieve specific experimental goals. Mouse [normal or CFTR(-/-)] airway epithelial cells are isolated and cultured by analogous techniques. Normal and CF epithelial cell lines that express the essential characteristics of the native tissue are obtained, cultured, and provided to SCOR investigators as needed to meet overall experimental goals. These activities reflect the centralized provision of established techniques that have successfully supported CF research for more than eight years, and the adaptation of newer reagents to this mission.

组织培养研究核心支持囊性纤维化中的 SCOR 研究人员通过提供来自人类的培养上皮细胞和 动物在适合计划实验的条件下进行。 这 总体目标通过四个具体目标来实现：1) 组织 获得; 2）患者特征； 3）原代细胞培养；和 4) 细胞系培养。 正常和囊性纤维化（CF 患者预定 选择性鼻或肺手术的确定和特征是 建立了临床和实验室标准。 上皮细胞是 从多余的切除组织中分离并通过建立的培养 保留必要的生理特性的技术 到拟议的研究。 专门的细胞纯化和培养 根据需要使用技术来实现特定的实验目标。 分离小鼠[正常或CFTR(-/-)]气道上皮细胞并 通过类似技术培养。 正常和 CF 上皮细胞系 表达天然组织的基本特征的是 根据需要获得、培养并提供给 SCOR 研究人员以满足 总体实验目标。 这些活动体现了中央集权 提供已成功支持 CF 的成熟技术 八年多的研究，以及更新试剂的适应 为了这个使命。