CORE--TISSUE CULTURE

核心——组织培养

• 批准号: 6496774
• 负责人: JAMES R YANKASKAS
• 金额: $ 24.35万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6496774
• 关键词: biomedical facility; cystic fibrosis; respiratory epithelium; tissue /cell culture

Epithelial cell cultures are instrumental in defining the pathophysiology of CF. A Tissue Procurement and Cell Culture Core was established at the University of North Carolina (UNC) in 1984, under the auspices of the Cystic Fibrosis Foundation, to provide standardized cell cultures to CF researchers. The Core has supported projects of the UNC Cystic Fibrosis Research Center since 1988, and has increased its output and capabilities to meet growing research demands. The Tissue Culture Core will support the SCOR projects through three major functions. 1. Tissue procurement. . Obtain nasal and lung tissue from normal, disease control, and cystic fibrosis humans as sources of airway epithelial cells. . Characterize donors with respect to diagnosis, demographic, and clinical features essential to research uses. 2. Airway epithelial cell isolation and culture. . Isolate epithelial cells from large and small airways of human, primate and rodent lungs. . Prepare and maintain primary epithelial cell cultures from define airway regions. . Prepare differentiated cultures from passaged, or cryopreserved and thawed cells on substrates as dictated by investigator needs. . Prepared and provide airway epithelial cell lines from gene knockout mice. 3. Cell culture technical development. . Optimize epithelial culture conditions to replicate the gene expression, morphological differentiation, and ion and mucus secretory functions of normal and CF large and small airways. . Produce genetically manipulated, well-differentiated primary airway epithelial cell cultures. . Provide very high humidity culture environments needed to assess airway surface liquid topography in the absence of evaporation artifacts.

上皮细胞培养有助于确定病理生理学 CF的。组织采购和细胞培养中心建立在 北卡罗来纳州大学（北卡罗来纳州），1984年，在 囊性纤维化基金会，为CF提供标准化细胞培养物 研究人员该中心支持了《囊性纤维化》杂志的项目。 研究中心自1988年以来，并已增加其产量和能力 以满足日益增长的研究需求。组织培养核心将支持 SCOR项目通过三个主要功能。 1.组织采集。 .从正常、疾病控制和囊性病变中获取鼻和肺组织 纤维化人类作为气道上皮细胞的来源。 .在诊断、人口统计学和临床方面表征供体 对研究用途至关重要的功能。 2.气道上皮细胞分离和培养。 .从人、灵长类动物的大气道和小气道分离上皮细胞 和啮齿动物的肺 .制备并维持原代上皮细胞培养物， 气道区域。 .从传代或冷冻保存的细胞中制备分化培养物， 根据研究者的需要，在基质上解冻细胞。 .制备并提供来自基因敲除的气道上皮细胞系 小鼠 3.细胞培养技术开发。 .优化上皮细胞培养条件以复制基因表达， 形态分化，离子和粘液分泌功能， 正常和CF大小气道。 .产生基因操纵的、分化良好的主气道 上皮细胞培养物。 .提供评估气道所需的高湿度培养环境 在没有蒸发伪影的情况下的表面液体形貌。