CORE--CELL CULTURE

细胞培养核心支持囊性纤维化的基因治疗 研究人员通过提供培养的上皮细胞和细胞系， 人类和动物在适合计划实验的条件下。 这一总体目标通过四个具体目标实现：1）组织 采集; 2）患者表征; 3）原代细胞培养;和4） 细胞系开发和培养。正常和囊性纤维化（CF） 确定计划进行选择性鼻或肺手术的患者， 以已建立的临床和实验室标准为特征。上皮 从过量切除的组织中分离细胞，并通过建立的 这些技术保留了人体所必需的生理特性 在拟议的研究中。专门的细胞纯化和培养 根据需要使用技术来实现特定的实验目标。 犬和小鼠[正常或cftr（-/-）]气道上皮细胞是 通过类似的技术分离和培养。上皮细胞系， 表达天然组织的基本特征 或获得，并根据需要提供给PPG研究者，以满足总体 实验目标。这些活动反映了集中提供 已建立的技术，已成功地支持CF研究， 超过9年，并适应新的试剂，以实现这些目标。

The Cell Culture Core supports Gene Therapy for Cystic Fibrosis investigators by supplying cultured epithelial cells and cell lines from humans and animals in conditions appropriate to the planned experiments. This overall goal is achieved through four Specific Aims: 1) tissue acquisition; 2) patient characterization; 3) primary cell culture; and 4) cell line development and culture. Normal and cystic fibrosis (CF) patients scheduled for elective nasal or lung surgery are identified and characterized by established clinical and laboratory criteria. Epithelial cells are isolated from excess excised tissues and cultured by established techniques that preserve the physiological properties that are essential to the proposed studies. Specialized cell purification and culture techniques are used as needed to achieve specific experimental goals. Canine and mouse [normal or cftr(-/-)] airway epithelial cells are isolated and cultured by analogous techniques. Epithelial cell lines that express the essential characteristics of the native tissue are developed or obtained, and provided to PPG investigators as needed to meet overall experimental goals. These activities reflect the centralized provision of established techniques that have successfully supported CF research for more than 9 years, and the adaptation of newer reagents to these goals.