EXTRACELLULAR MATRIX INTERACTIONS IN CARDIAC MORPHOGENESIS
EXTRACELLULAR MATRIX INTERACTIONS IN CARDIAC MORPHOGENESIS
批准号:
6242045
负责人:
MICHAEL SOLURSH
金额:
$16.91万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-01-01 至 1997-12-31
关键词:
animal genetic material tag blocking antibody cardiovascular disorder epidemiology cell cell interaction cell differentiation cell migration chick embryo collagen congenital heart disorder early embryonic stage elastin extracellular matrix fibronectins heart cell heart function heart valves histogenesis homeobox genes hyaluronate immunocytochemistry in situ hybridization laboratory mouse laboratory rat laminin molecular genetics myosins tissue /cell culture
中文摘要
很大比例的先天性心脏缺陷见于瓣膜和
英文摘要
A large proportion of congenital heart defects are seen in the valves and
membranous septa of the heart, which are in part derived from the
endocardial cushions. The morphogenesis of the endocardial cushions is a
complex process, involving a sequence of basic developmental events. These
include cell migration, proliferation, and differentiation. It is the goal
of this project to define this developmental sequence in greater detail
and to understand the role of specific genes in the process. The major
focus is on components of the extracellular matrix. The following
questions will be addressed. l) What is the role of the homeobox gene, Hox
7, in the initiation of cushion morphogenesis? This will be addressed by
examining the effects of anti-sense deoxyoligonucleotides on cushion
morphogenesis and on the expression of extracellular matrix components in
heart explant cultures and in ovo. 2) What extracellular matrix molecules
are expressed during cushion formation? This will be addressed by
immunohistochemistry with a battery of antibodies available in the
laboratory and by in situ hybridization in some cases of special interest.
3) What is the developmental effect of blocking the expression of specific
extracellular matrix components on cushion morphogenesis? This will be
carried out with blocking antibodies, where available, or anti-sense
deoxyoligonucleotides. In particular we will focus on hyaluronan and its
binding proteins (CD-44 and PG-M), collagen II, and a component of the
cardiac jelly (1E12) which has been identified recently in this
laboratory. 4) The role of other genes in cushion morphogenesis will be
studied subsequently, as they become characterized. 5) What is the
contribution of the cushion cells to the tissues and extracellular matrix
of the mature valves and septa of the heart? This question will be
addressed by immunohistological analysis after in situ labeling of cushion
cells with replication-defective, lac-Z labeled retrovirus, followed by
the reincubation of the windowed, injected chicken eggs.
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EXTRACELLULAR MATRIX INTERACTIONS IN CARDIAC MORPHOGENESIS
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批准号:6109979
-
项目类别:
-
资助金额:$17.73万
-
财政年份:1998
-
负责人:MICHAEL SOLURSH
-
依托单位:
PHOSPHORIMAGING SYSTEM
-
批准号:2283879
-
项目类别:
-
资助金额:$9.5万
-
财政年份:1993
-
负责人:MICHAEL SOLURSH
-
依托单位:
CONFOCAL LASER SCANNING MICROSCOPE
-
批准号:3520622
-
项目类别:
-
资助金额:$15.4万
-
财政年份:1990
-
负责人:MICHAEL SOLURSH
-
依托单位:
CELL--ECM INTERACTIONS IN MYOCARDIAL FORM AND FUNCTION
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批准号:3362274
-
项目类别:
-
资助金额:$8.35万
-
财政年份:1989
-
负责人:MICHAEL SOLURSH
-
依托单位:
GENES IMPORTANCE IN CRANIOFACIAL ANOMALIES
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批准号:3105769
-
项目类别:
-
资助金额:$0.49万
-
财政年份:1989
-
负责人:MICHAEL SOLURSH
-
依托单位:
CELL--ECM INTERACTIONS IN MYOCARDIAL FORM AND FUNCTION
-
批准号:3362275
-
项目类别:
-
资助金额:$8.68万
-
财政年份:1989
-
负责人:MICHAEL SOLURSH
-
依托单位:
GENES IMPORTANCE IN CRANIOFACIAL ANOMALIES
-
批准号:3105772
-
项目类别:
-
资助金额:$60.15万
-
财政年份:1989
-
负责人:MICHAEL SOLURSH
-
依托单位:
CELL--ECM INTERACTIONS IN MYOCARDIAL FORM AND FUNCTION
-
批准号:3362272
-
项目类别:
-
资助金额:$10.87万
-
财政年份:1989
-
负责人:MICHAEL SOLURSH
-
依托单位:
GENES IMPORTANT IN CRANIOFACIAL ANOMALIES
-
批准号:3105773
-
项目类别:
-
资助金额:$63.35万
-
财政年份:1989
-
负责人:MICHAEL SOLURSH
-
依托单位:
GENES IMPORTANCE IN CRANIOFACIAL ANOMALIES
-
批准号:3105770
-
项目类别:
-
资助金额:$58.18万
-
财政年份:1989
-
负责人:MICHAEL SOLURSH
-
依托单位:
CELL--ECM INTERACTIONS IN MYOCARDIAL FORM AND FUNCTION
-
批准号:2221090
-
项目类别:
-
资助金额:$9.09万
-
财政年份:1989
-
负责人:MICHAEL SOLURSH
-
依托单位:
GENES IMPORTANCE IN CRANIOFACIAL ANOMALIES
-
批准号:3105771
-
项目类别:
-
资助金额:$57.76万
-
财政年份:1989
-
负责人:MICHAEL SOLURSH
-
依托单位:
CELL--ECM INTERACTIONS IN MYOCARDIAL FORM AND FUNCTION
-
批准号:3362273
-
项目类别:
-
资助金额:$7.98万
-
财政年份:1989
-
负责人:MICHAEL SOLURSH
-
依托单位:
GENES IMPORTANCE IN CRANIOFACIAL ANOMALIES
-
批准号:3105768
-
项目类别:
-
资助金额:$55.45万
-
财政年份:1989
-
负责人:MICHAEL SOLURSH
-
依托单位:
BIOLOGY OF DEVELOPING SYSTEMS
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批准号:3539207
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项目类别:
-
资助金额:$5.05万
-
财政年份:1981
-
负责人:MICHAEL SOLURSH
-
依托单位:
THE BIOLOGY OF DEVELOPING SYSTEMS
-
批准号:3539205
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项目类别:
-
资助金额:$4.15万
-
财政年份:1981
-
负责人:MICHAEL SOLURSH
-
依托单位:
THE BIOLOGY OF DEVELOPING SYSTEMS
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批准号:3539208
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项目类别:
-
资助金额:$5.0万
-
财政年份:1981
-
负责人:MICHAEL SOLURSH
-
依托单位:
THE BIOLOGY OF DEVELOPING SYSTEMS
-
批准号:3539206
-
项目类别:
-
资助金额:$4.44万
-
财政年份:1981
-
负责人:MICHAEL SOLURSH
-
依托单位:
THE BIOLOGY OF DEVELOPING SYSTEMS
-
批准号:3539204
-
项目类别:
-
资助金额:$4.41万
-
财政年份:1981
-
负责人:MICHAEL SOLURSH
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依托单位:
REGULATION OF LIMB CHONDROGENESIS
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批准号:3310395
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项目类别:
-
资助金额:$24.4万
-
财政年份:1978
-
负责人:MICHAEL SOLURSH
-
依托单位:
海外基金