CORE--FLUORESCENCE MICROSCOPY

此核心的主要目标是为以下对象提供成像分析工具 该计划项目的研究人员。核心助力 需要专业知识和专门设备的实验不需要 可在各个分项目实验室中使用。该委员会的成员 CORE II将为不同成员提供指导和专家帮助 在核心中可用的各种成像方法中的PMI II和正在开发的相关微观设施中 各种共享的仪器赠与。CORE II还用于培训 PMI成员的博士后和博士后研究员在使用 显微镜。第二核心的这一培训作用将继续是 高优先级。核心现在和拟议赠款的期限内 课程将重点放在荧光探针的定量分析上 在活细胞内。这些方法包括视频显微镜、3-D 共聚焦图像重建、细胞运动分析、荧光 笼状化合物的偏振、光活化、弱光 检测方法和免疫荧光。酷睿II还拥有 活细胞微量注射设备，未标记和已标记 含有不同细胞骨架的cDNA的蛋白质和质粒 蛋白质。

The major aim of this Core is to provide an imaging analysis facility for the investigators of this Program Project. The Core assists in experiments that require expertise and specialized equipment not available in the individual subproject laboratories. The members of the Core II will provide guidance and expert help to the different members of the PMI in the various imaging methods that are available in the Core II and in the associated microscopic facilities being developed through the various Shared Instrument grants. Core II also serves to train postdoctoral and predoctoral fellows of PMI members in the use of microscopes. This training role of the Core II will continue to be a high priority. The Core now and for the duration of the proposed grant period will be focusing on quantitative analysis of fluorescent probes inside living cells. The approaches include videomicroscopy, 3-D reconstruction of confocal images, cell motility assays, fluorescence polarization, photoactivation of caged compounds, low light level detection methods, and immunofluorescence. Core II also possesses the equipment for microinjecting, into living cells, unlabeled and labeled proteins as well as plasmids containing cDNAs for different cytoskeletal proteins.