Purchase of Deconvolution Microscope System

描述（由申请人提供）：一组细胞和发育生物学家要求为去卷积显微镜的共享设施提供资金。该提案的目的是提供一个最先进的系统，其中显微镜，解卷积软件，计算机，相机和环境室集成在各种生物体的亚细胞结构的最佳成像。该设施计划提供对活细胞和固定细胞进行延时研究的能力。FRAP能力被要求用于研究表达GFP标记蛋白的细胞中蛋白质的动力学。申请人通常使用共聚焦显微镜，但对于他们研究的某些方面，去卷积可能增加的信噪比将允许检测用其标本的共聚焦图像不可行的实验细节。去卷积显微镜系统将为所有这些生物学家开辟令人兴奋的新可能性。

DESCRIPTION (provided by applicant): A group of cell and developmental biologists requests funds for a shared facility for deconvolution microscopy. The aim of the proposal is to provide a state-of-the-art system in which microscope, deconvolution software, computer, cameras, and environmental chamber are integrated for optimal imaging of subcellular structures in a variety of organisms. The facility is planned to provide capability for time-lapse study of live cells as well as fixed cells. FRAP capability is requested for studies of the dynamics of proteins in cells expressing GFP- tagged proteins. The applicants routinely use confocal microscopy, but for some aspects of their studies, the increased signal-to-noise possible with deconvolution will allow detection of experimental details not feasible with confocal images of their specimens. A deconvolution microscope system would open up exciting new possibilities for all of these biologists.