Purchase of Deconvolution Microscope System

描述（由申请人提供）：一组细胞和发育生物学家申请资金用于反褶积显微镜共用设备。该提案的目的是提供一个最先进的系统，其中显微镜、反褶积软件、计算机、相机和环境室集成在一起，以实现各种生物亚细胞结构的最佳成像。该设施计划提供活细胞和固定细胞的延时研究能力。在表达GFP标记蛋白的细胞中，需要FRAP能力来研究蛋白质的动力学。申请人通常使用共聚焦显微镜，但对于他们研究的某些方面，反卷积可能增加的信噪比将允许检测实验细节，而这些细节在其标本的共聚焦图像中是不可行的。反褶积显微镜系统将为所有这些生物学家开辟令人兴奋的新可能性。

DESCRIPTION (provided by applicant): A group of cell and developmental biologists requests funds for a shared facility for deconvolution microscopy. The aim of the proposal is to provide a state-of-the-art system in which microscope, deconvolution software, computer, cameras, and environmental chamber are integrated for optimal imaging of subcellular structures in a variety of organisms. The facility is planned to provide capability for time-lapse study of live cells as well as fixed cells. FRAP capability is requested for studies of the dynamics of proteins in cells expressing GFP- tagged proteins. The applicants routinely use confocal microscopy, but for some aspects of their studies, the increased signal-to-noise possible with deconvolution will allow detection of experimental details not feasible with confocal images of their specimens. A deconvolution microscope system would open up exciting new possibilities for all of these biologists.