Purchase of Deconvolution Microscope System

购买反卷积显微镜系统

• 批准号: 6581799
• 负责人: Joseph William Sanger
• 金额: $ 41.92万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6581799
• 关键词: biomedical equipment purchase; cell biology; green fluorescent proteins; microscopy; structural biology

DESCRIPTION (provided by applicant): A group of cell and developmental biologists requests funds for a shared facility for deconvolution microscopy. The aim of the proposal is to provide a state-of-the-art system in which microscope, deconvolution software, computer, cameras, and environmental chamber are integrated for optimal imaging of subcellular structures in a variety of organisms. The facility is planned to provide capability for time-lapse study of live cells as well as fixed cells. FRAP capability is requested for studies of the dynamics of proteins in cells expressing GFP- tagged proteins. The applicants routinely use confocal microscopy, but for some aspects of their studies, the increased signal-to-noise possible with deconvolution will allow detection of experimental details not feasible with confocal images of their specimens. A deconvolution microscope system would open up exciting new possibilities for all of these biologists.

描述(由申请者提供)：一组细胞和发育生物学家申请资金用于一个共用的去卷积显微镜设备。该提案的目的是提供一种最先进的系统，其中集成了显微镜、去卷积软件、计算机、照相机和环境室，以便对各种生物体的亚细胞结构进行最佳成像。该设施计划提供对活细胞和固定细胞进行延时研究的能力。FRAP功能是研究细胞中表达GFP标记蛋白的蛋白质的动力学所必需的。申请者通常使用共聚焦显微镜，但对于他们研究的某些方面，通过去卷积可能增加的信噪比将允许利用其标本的共聚焦图像来检测实验细节。去卷积显微镜系统将为所有这些生物学家开辟令人兴奋的新可能性。