LIFETIME IMAGING OF Z-BAND PROTEIN IN LIVING MUSCLE CELLS

活体肌肉细胞中 Z 带蛋白的终生成像

• 批准号: 7373155
• 负责人: Joseph William Sanger
• 金额: $ 0.68万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7373155
• 关键词: LIFETIME; IMAGING; BAND; PROTEIN; LIVING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our lab specializes in fluorescence imaging of Z-band proteins during development of skeletal and cardiac muscle cells. Currently we are applying intensity-based FRET-imaging techniques to cells expressing CFP and YFP fusions with several Z-band proteins. (alpha-actinin, myozenin, telethonin) in an attempt to better understand their changing arrangement during development. Because artifacts and concentration effects make interpretation of steady-state data difficult, we want to apply lifetime imaging to get less ambiguous measurements of FRET efficiencies for several donor-acceptor paris. We also hope to be able to compare FRET efficiencies between pairs and between cells imaged at different development stages of the cells.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。我们的实验室专门研究骨骼肌和心肌细胞发育过程中 Z 带蛋白的荧光成像。目前，我们正在将基于强度的 FRET 成像技术应用于表达 CFP 和 YFP 与几种 Z 带蛋白融合的细胞。 （α-肌动蛋白、myozenin、telethonin），试图更好地了解它们在发育过程中变化的排列。由于伪影和浓度效应使得稳态数据的解释变得困难，我们希望应用寿命成像来获得几个供体-受体巴黎的 FRET 效率的不太模糊的测量结果。我们还希望能够比较细胞对之间以及在细胞不同发育阶段成像的细胞之间的 FRET 效率。