LIFETIME IMAGING OF Z-BAND PROTEIN IN LIVING MUSCLE CELLS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们的实验室专门研究骨骼和心肌细胞发育过程中Z-带蛋白的荧光成像。目前，我们正在将基于强度的FRET成像技术应用于表达CFP和YFP与几个Z-带蛋白融合的细胞。(α-肌动蛋白、肌动蛋白、端粒素)，试图更好地了解它们在发育过程中排列的变化。由于伪影和浓度效应使得对稳态数据的解释变得困难，我们想要应用寿命成像来获得几个供体-受体巴黎的FRET效率的不那么模糊的测量。我们还希望能够比较不同细胞对之间以及不同发育阶段的细胞之间的FRET效率。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our lab specializes in fluorescence imaging of Z-band proteins during development of skeletal and cardiac muscle cells. Currently we are applying intensity-based FRET-imaging techniques to cells expressing CFP and YFP fusions with several Z-band proteins. (alpha-actinin, myozenin, telethonin) in an attempt to better understand their changing arrangement during development. Because artifacts and concentration effects make interpretation of steady-state data difficult, we want to apply lifetime imaging to get less ambiguous measurements of FRET efficiencies for several donor-acceptor paris. We also hope to be able to compare FRET efficiencies between pairs and between cells imaged at different development stages of the cells.