PHLEBOTOMY OF NORMAL VOLUNTEERS TO SUPPORT PLASMODIUM FALCIPARUM

每周从健康志愿者那里收集的红细胞已经被用于 维持疟疾寄生虫（疟原虫）的体外培养 恶性疟原虫）。 这些微生物已被用于一系列实验室 问题研究 一个方面需要抗疟药物的体外评价， 青蒿素合成类似物的效力。 从这些研究中， 获得了新的见解的分子机制的行动， 重要的一类代理人。 培养也被用来分离DNA 和恶性疟原虫的RNA，以监测热休克的产生， 蛋白质，以感染蚊子，并在研究旨在评估 候选疫苗 最后，这些培养物被用来感染 在一项挑战研究中使用的蚊子， 阿托喹酮可以预防人类疟疾。

Red blood cells collected weekly from healthy volunteers have been used to sustain in vitro cultures of malaria parasites (Plasmodium falciparum). These organisms have been used in an array of laboratory studies. One aspect entails the in vitro evaluation of antimalarial potency of synthetic analogs of artemisinin. From these studies we have gained new insights into the molecular mechanism of action of this important class of agents. Cultures have also been used to isolate DNA and RNA from P. falciparum, to monitor the production of heat shock proteins, to infect mosquitoes, and in studies designed to evaluate vaccine candidates. Finally, these cultures were used to infect mosquitoes that were used in a challenge study to determine whether atoquavone could prevent malaria in humans.