INTERFACIAL ADSORPTION OF PULMONARY SURFACTANT

肺表面活性剂的界面吸附

• 批准号: 2430562
• 负责人: STEPHEN B HALL
• 金额: $ 7.02万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2430562
• 关键词: adsorption; animal extract; biophysics; fatty acylation; high performance liquid chromatography; lung; mathematical model; molecular film; palmitates; phosphatidylcholines; pulmonary surfactants; surface property; thermodynamics; thin layer chromatography

This application requests support to provide a substantial increase in the principal investigator's protected time for research on pulmonary surfactant. The principal investigator (PI) brings to his research a combination of solid training in both physical science and clinical medicine, including a doctorate in biophysical chemistry and a fellowship in pulmonary and critical care medicine. He is currently an assistant professor with extensive clinical, teaching, and administrative responsibilities in addition to his research. He now has eight years of experience in research concerning pulmonary surfactant, including four years as an independent investigator in his own laboratory. These studies have been supported by several grants designed for starting investigators. He currently is PI or co-PI on three peer-reviewed nationally competitive grants, including two from the Whitaker Foundation and a recent First Award from NIH. His research focuses on two critical steps essential for surfactant function. The studies proposed in this application will examine mechanisms by which pulmonary surfactant adsorbs rapidly to form interfacial films. A second project concerns structures within the surface films allow them to remain stable at the interface during compression to very low surface tensions. A manuscript concerning preliminary findings is approaching submission, and specific aims are being formulated for an application to provide grant support. The PI's research emphasizes collaboration with other laboratories which provide access to state-of-the- art methods in interfacial science which have not previously been used to study pulmonary surfactant. The ultimate goal of the PI's research is to extend the successful use of surfactant in neonates to adult patients, and in particular to develop inexpensive effective surfactant to i=minimize the cost of the larger doses required in adults. The funds provided by this grant would increase the PI's commitment to research from 45% to 83%. His only other obligations would be 1-2 seminars and 102 lectures per year on subjects directly relate to his research, and 8 weeks of clinical responsibility which he has requested to maintain his clinical skills. The research proposal is the project funded by a First Award which began one month prior to the submission of the application. The proposed studies will establish mechanisms by which pulmonary surfactant adsorbs rapidly to an air-liquid interface to form interfacial films capable of reducing surface tension during compression.

此应用程序请求支持以提供大幅增加的 首席研究员对肺部疾病研究的保护时间 表面活性剂。首席调查员(PI)为他的研究带来了 扎扎实实的体能训练与临床训练相结合 医学，包括生物物理化学博士学位和奖学金 在肺部和重症监护医学方面。他目前是一名助理 具有丰富的临床、教学和管理经验的教授 除了他的研究之外，他还承担着其他责任。他现在有八年的时间 肺表面活性物质研究的经验，包括四个 在自己的实验室里当了几年的独立调查员。这些研究 已经得到了为启动调查人员而设计的几笔赠款的支持。 他目前是三个同行评议的国家竞争对手的PI或共同PI 赠款，包括惠特克基金会的两项拨款和最近的一项第一奖 来自美国国立卫生研究院。他的研究集中于两个关键步骤，这两个步骤对于 表面活性物质的作用。本申请书中提出的研究将审查 肺表面活性物质快速吸附形成的机制 界面膜。第二个项目涉及地表内部的结构。 薄膜允许它们在压缩过程中在界面上保持稳定 表面张力非常低。关于初步发现的手稿是 正在接近提交，正在为一个 申请提供赠款支持。国际和平研究所的研究强调 与其他实验室合作，这些实验室提供访问最新技术的途径 界面科学中以前从未使用过的艺术方法 研究肺表面活性物质。国际和平研究所研究的最终目标是 将表面活性物质在新生儿中的成功应用推广到成年患者，以及 特别是开发廉价有效的表面活性剂，使I=最大限度地减少 成人所需的较大剂量的费用。由该公司提供的资金 格兰特将把PI对研究的承诺从45%提高到83%。他的 唯一的其他义务是每年1-2次研讨会和102次讲座 与他的研究直接相关的科目，以及8周的临床 这是他要求的责任，以保持他的临床技能。 研究提案是由第一个奖项资助的项目，该奖项始于 在提交申请前一个月。建议进行的研究 将建立肺表面活性物质快速吸附到 形成能够还原的界面膜的气-液界面 压缩过程中的表面张力。