SOFTWARE AND INSTRUMENTATION FOR MAPPING AND SEQUENCING

该提案将允许申请人利用其工程和 编程背景，以推进软件和仪器 基因组绘图和测序所需的技术， 有能力进行独立研究。 三个具体领域 重点是： L.继续开发物理绘图程序SEGMAP， 结合利用从RARE获得的STS间隔数据的算法 裂解反应 2.人和小鼠T细胞受体基因座的序列分析 为了更好地理解进化的发展， 这个家族基因的结构和功能。 3.开发仪器，以实现 规划和排序项目;第一项工作将是开发， 测试自动凝胶读取器。 这些发展努力将通过独特的跨学科 分子生物技术部提供的研究机会。在 华盛顿大学。 此外，每个顾问都是 各自领域的专家：菲利普绿色（基因组数据分析）， Leroy Hood（DNA测序和免疫系统遗传学），Maynard Olson （基因组作图）和格尔货车登恩（生物仪器 发展）。 申请人具有空气动力学研究背景， 在自动化工作、硬件集成、数据 采集、分析和编程。 这些地区非常适合 开发基因组过程自动化和数据分析的任务 作图和测序。

This proposal will allow the applicant to utilize his engineering and programming background to advance software and instrumentation technologies necessary for genome mapping and sequencing and to develop the capability to carry out independent research. Three specific areas of focus are: l. Continued development of the physical mapping program, SEGMAP, by incorporating algorithms to utilize STS spacing data obtained from RARE cleavage reactions. 2. Analysis of sequence data from T-cell receptor loci in human and mouse in order to better understand the evolutionary development, and the structure a,nd, function of the genes in this family. 3. Development of instrumentation to automate procedures utilized in mapping and sequencing projects; the first effort will be development and testing of an automated gel reader. These development efforts will be enhanced by the unique interdisciplinary research opportunities presented by the Molecular Biotechnology Dept. at the University of Washington. Furthermore, each of the advisors are expert in their respective fields: Philip Green (genome data analysis), Leroy Hood (DNA sequencing and immune system genetics), Maynard Olson (genome mapping), and Ger Van Den Engh (biological instrumentation development). The applicant has a background in aerodynamic research with extensive experience in automation efforts, hardware integration, data acquisition and analysis, and programming. These areas are well suited to the task of developing process automation and data analysis for genome mapping and sequencing.