SOFTWARE AND INSTRUMENTATION FOR MAPPING AND SEQUENCING

用于绘图和测序的软件和仪器

• 批准号: 2609160
• 负责人: CHARLES L MAGNESS
• 金额: $ 11.27万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2609160
• 关键词: computer assisted sequence analysis; computer program /software; genetic mapping; nucleic acid sequence; nucleic acid structure

This proposal will allow the applicant to utilize his engineering and programming background to advance software and instrumentation technologies necessary for genome mapping and sequencing and to develop the capability to carry out independent research. Three specific areas of focus are: l. Continued development of the physical mapping program, SEGMAP, by incorporating algorithms to utilize STS spacing data obtained from RARE cleavage reactions. 2. Analysis of sequence data from T-cell receptor loci in human and mouse in order to better understand the evolutionary development, and the structure a,nd, function of the genes in this family. 3. Development of instrumentation to automate procedures utilized in mapping and sequencing projects; the first effort will be development and testing of an automated gel reader. These development efforts will be enhanced by the unique interdisciplinary research opportunities presented by the Molecular Biotechnology Dept. at the University of Washington. Furthermore, each of the advisors are expert in their respective fields: Philip Green (genome data analysis), Leroy Hood (DNA sequencing and immune system genetics), Maynard Olson (genome mapping), and Ger Van Den Engh (biological instrumentation development). The applicant has a background in aerodynamic research with extensive experience in automation efforts, hardware integration, data acquisition and analysis, and programming. These areas are well suited to the task of developing process automation and data analysis for genome mapping and sequencing.

这项提案将允许申请人利用他的工程和 高级软件和工具的编程背景 基因组图谱和测序所需的技术以及开发 进行独立研究的能力。以下三个具体领域 重点是： L。继续发展物理测绘计划，SEGMAP，由 整合算法以利用从REARE获得的STS间距数据 裂解反应。 2.人和小鼠T细胞受体基因座序列数据分析 为了更好地理解进化的发展，以及 这个家族中基因的结构和功能。 3.开发仪表化工具以自动化在 测绘和测序项目；第一项工作将是开发和 自动凝胶阅读器的测试。 这些发展努力将通过独特的跨学科得到加强 分子生物技术系提供的研究机会。在… 华盛顿大学。此外，每个顾问都是 各自领域的专家：菲利普·格林(基因组数据分析)， 勒罗伊·胡德(DNA测序和免疫系统遗传学)，梅纳德·奥尔森 (基因组图谱)和Ger Van Den Engh(生物仪器) 发展)。申请者有空气动力学研究的背景。 在自动化、硬件集成、数据方面拥有丰富的经验 采集和分析，以及编程。这些地区非常适合 开发基因组过程自动化和数据分析的任务 测绘和测序。