CORE--LABORATORY SERVICES

核心——实验室服务

• 批准号: 6239525
• 负责人: BRIAN BUSH
• 金额: $ 20.71万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT ALBANY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 1998-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6239525
• 关键词: analytical chemistry; atomic absorption spectrometry; biomedical facility; dosage; endocrinology; environmental contamination; halobiphenyl /halotriphenyl compound; laboratory rat; mass spectrometry; metal poisoning; radioimmunoassay

The laboratory Services Core will be concerned with three series of analysis, PCB, endocrine, and metals, also with the care and dosing of pregnant rats precisely-timed which will be employed by the four biomedical projects. PCB analysis will be by gas chromatography with electron capture detection using two capillary columns a silicone stationary phase (Ultra II) and an hydrocarbon stationary phase (Apiezon L); the latter will be used for peak confirmation. Human serum will be the commonest analytical matrix, experimental animal tissues, water, air and dust will also be analyzed for PCB, selected samples will be analyzed for 12 coplanar and semicoplanar PCB after Hypercarb HPLC separation and for PCDF's after further clean up using mass spectrometry. Endocrine analysis will be undertaken by standard RIA tests on human samples and by modified immunological tests for animals. Lead, organic and inorganic mercury and fluoride will be determined in children's samples by atomic absorption spectroscopy and ion selective electrode by the Centre de Toxicologie du Quebec under contract. Pregnant precisely-timed rats will be obtained from a reliable supplier and dosed with Aroclor 1248, the pollutant at Akwesasne and four individual PCB congeners representing various classes of PCB with differing biologic activity, namely; 2,4,4'-trichlorobiphenyl (#28)2,4,5,3',4'- pentachlorobiphenyl(#118) 3,4,5,3',4'-pentachlorobiphenyl(#126) 2,4,5,2',4',5'-hexachlorobiphenyl(#153). These dams and their pups will be used for different experiments in Projects 3-6. Novel organic chemical analysis will be provided using GC/FTIR/MS high resolution mass spectrometry and HPLC/CI/MS for metabolites and degradation products derived from the 4 biomedical and three degradation projects. This core project will involve a large amount of data collation and preliminary assembly in service of both Core B and the other 10 projects.

实验室服务核心将关注三个系列 分析、多氯联苯、内分泌和金属，也与护理和剂量 怀孕的大鼠将被四家生物医学公司雇用 项目。 多氯联苯的分析将通过带有电子捕获检测的气相色谱进行 使用两个毛细管柱，硅胶固定相(Ultra II)和 烃类固定相(阿皮松L)；后者将用于峰 确认。人类血清将成为最常见的分析矩阵， 实验动物组织、水、空气和灰尘也将被分析 印刷电路板，选定的样品将分析12个共面和半平面印刷电路板 Hypercarb高效液相色谱分离和PCDF进一步净化后使用 质谱学。内分泌分析将由标准RIA进行 对人类样本的测试和对动物的改良免疫学测试。 铅、有机和无机汞和氟化物将在#年测定。 原子吸收光谱分析和离子选择性测定儿童样品 电极由魁北克毒理中心签约。 怀孕的大鼠将从可靠的供应商获得准确的时间和 使用Aroclor 1248，Akwesasne的污染物和四个单独的多氯联苯 代表具有不同生物的不同类别的多氯联苯的同系物 活性，即：2，4，4‘-三氯联苯(#28)2，4，5，3’，4‘- 五氯联苯(#118)3，4，5，3‘，4’-五氯联苯(#126) 2，4，5，2‘，4’，5‘-六氯联苯(#153)。这些水坝和它们的幼崽 用于项目3-6中的不同实验。 将利用GC/FTIR/MS HIGH提供新的有机化学分析 代谢产物的解析质谱学和高效液相色谱-电离质谱联用分析 来自4个生物医学项目和3个降解项目的产品。 这个核心项目将涉及大量的数据整理和 为核心B和其他10个项目服务的初步组装。