CORE--LABORATORY SERVICES

核心——实验室服务

• 批准号: 6504074
• 负责人: BRIAN BUSH
• 金额: $ 23.08万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT ALBANY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6504074
• 关键词: analytical chemistry; atomic absorption spectrometry; biomedical facility; dosage; endocrinology; environmental contamination; halobiphenyl /halotriphenyl compound; laboratory rat; mass spectrometry; metal poisoning; radioimmunoassay

The laboratory Services Core will be concerned with three series of analysis, PCB, endocrine, and metals, also with the care and dosing of pregnant rats precisely-timed which will be employed by the four biomedical projects. PCB analysis will be by gas chromatography with electron capture detection using two capillary columns a silicone stationary phase (Ultra II) and an hydrocarbon stationary phase (Apiezon L); the latter will be used for peak confirmation. Human serum will be the commonest analytical matrix, experimental animal tissues, water, air and dust will also be analyzed for PCB, selected samples will be analyzed for 12 coplanar and semicoplanar PCB after Hypercarb HPLC separation and for PCDF's after further clean up using mass spectrometry. Endocrine analysis will be undertaken by standard RIA tests on human samples and by modified immunological tests for animals. Lead, organic and inorganic mercury and fluoride will be determined in children's samples by atomic absorption spectroscopy and ion selective electrode by the Centre de Toxicologie du Quebec under contract. Pregnant precisely-timed rats will be obtained from a reliable supplier and dosed with Aroclor 1248, the pollutant at Akwesasne and four individual PCB congeners representing various classes of PCB with differing biologic activity, namely; 2,4,4'-trichlorobiphenyl (#28)2,4,5,3',4'- pentachlorobiphenyl(#118) 3,4,5,3',4'-pentachlorobiphenyl(#126) 2,4,5,2',4',5'-hexachlorobiphenyl(#153). These dams and their pups will be used for different experiments in Projects 3-6. Novel organic chemical analysis will be provided using GC/FTIR/MS high resolution mass spectrometry and HPLC/CI/MS for metabolites and degradation products derived from the 4 biomedical and three degradation projects. This core project will involve a large amount of data collation and preliminary assembly in service of both Core B and the other 10 projects.

实验室服务核心将涉及三个系列， 分析，多氯联苯，内分泌，和金属，也与护理和剂量的 妊娠大鼠精确定时，将由四个生物医学 项目 多氯联苯分析将采用气相色谱法和电子捕获检测器 使用两个毛细管柱，硅胶固定相（Ultra II）和 烃固定相（Apiezon L）;后者将用于峰 确认. 人血清将是最常见的分析基质， 实验动物组织，水，空气和灰尘也将被分析， PCB，将对12个共面和半共面PCB的选定样本进行分析 在Hypercarb HPLC分离后，以及在使用 质谱分析法来 将通过标准RIA进行内分泌分析 通过对人类样本的测试和对动物的改良免疫学测试。 铅、有机和无机汞和氟化物将在 原子吸收光谱法和离子选择性 电极由魁北克毒理学中心根据合同提供。 将从可靠的供应商处获得精确定时的妊娠大鼠， 使用Aroclor 1248，Akwesasne的污染物和四个单独的PCB 代表具有不同生物特性的多氯联苯的各种同系物 活性，即：2，4，4 '-三氯联苯（#28）2，4，5，3'，4 '- 五氯联苯（#118）3，4，5，3 '，4'-五氯联苯（#126） 2，4，5，2 '，4'，5 '-六氯联苯（#153）。 这些母鼠和它们的幼崽 用于项目3-6中的不同实验。 新的有机化学分析将提供使用GC/FTIR/MS高 代谢产物和降解产物的分离度质谱和HPLC/CI/MS 产品来自4个生物医学和3个降解项目。 这个核心项目将涉及大量的数据整理， 为核心B和其他10个项目服务的初步组装。