STRUCTURE ELUCIDATION OF PROTEINS BY MASS SPECTROMETRY

在结构生物学研究所期间，史密斯教授将把他的时间分配给三个不同的活动。他大约三分之一的时间将用于休假的主要目标，利用X射线结晶学、核磁共振、冷冻电子显微镜和分子生物学与结构生物学家互动。他还将花费大约三分之一的时间共同指导一名研究生(弗洛雷斯坦·德斯马里斯)和一名博士后助理研究人员(让·阿门戈博士)的研究。这两家公司都使用HX MS检测蛋白质的构象变化，并由IBS蛋白质光谱分析实验室主任Eric Forest博士监督。这项活动预计将成为就HX MS方法的实验细节进行富有成效的交流的基础。史密斯教授剩余三分之一的努力将被要求指导他在内布拉斯加州大学正在进行的HX MS研究项目。这个研究小组由大约10名研究生、4名博士后助理研究员和3名长期工作人员组成，主要由两项NIH拨款资助(ROI GM 40384和RO1EY07609)。

While at the Institut de Biologie Structurale, Prof Smith will divide his time among three different activities. Approximately one third of his time will be devoted to the principal goal of the sabbatical leave, interacting with structural biologists using X-ray crystallography, nuclear magnetic resonance, cryo- electron microscopy and molecular biology. He will also spend approximately one third of his time co-directing the research of one graduate student (Florestan Desmaris) and one postdoctoral research associate (Dr. Jean Armengaud). Both use HX MS to detect conformational changes in proteins and are under the supervision of Dr. Eric Forest, Director to of the Laboratoire de Spectrometrie de Masse des Proteines at IBS. This activity is expected to be the basis for a fruitful exchange on experimental details of HX MS methodology. The remaining third of Prof. Smith's effort will be required to direct his on-going research program in HX MS at the University of Nebraska. This research group, which consists of approximately 10 graduate students, four postdoctoral research associates and three permanent staff, is funded principally by two NIH grants (ROI GM 40384 and RO1 EY07609).