CORE--BIOHAZARD CONTROL

核心——生物危害控制

• 批准号: 6353462
• 负责人: MOHAN SOMASUNDARAN
• 金额: $ 11.68万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6353462
• 关键词: AIDS; NOD mouse; SCID mouse; animal breeding; biohazard control; biomedical facility; cell line; flow cytometry; health science research; helper T lymphocyte; human immunodeficiency virus; macrophage; simian immunodeficiency virus

The UMass Biohazard Containment Core facility will be comprises of the Virology Core Facility and the Animal Biohazard facility and will operate has a centralized source of reagents for in vitro cell-culture systems and for in vivo animal systems to support the participating CFAR investigators. The Virology Core facility will provide well-characterized stocks of prototypic laboratory strains of primate immunodeficiency viruses. In addition, the facility will provide stocks of well-characterized patient isolates and stocks of viruses obtained from infectious molecular clones. The viral stocks generated in primary CD4 T cells, in monocyte-derived macrophage, or in T cell lines, will be titered and phenotypically characterized for syncytium-inducing properties, replication kinetics and cytopathic properties. Aliquots of the viral stocks will be archived in liquid nitrogen to ensure a constant supply of virus. The Virology Core facility will also supply primary CD4 T cells and macrophages for the CFAR investigators. A FACscan flow cytometer and a FACsort flow cytometer in the facility will be used to analyze cell surface markers and provide aseptically sorted cells. The Animal Biohazard facility will provide animals, technical support and expertise for investigators who wish to use SCID mouse in vivo model systems as a part of their AIDS research. The facility will maintain a BL2+ level of contain that will support a safe and biohazard free environment for work with pathogenic immunodeficient viruses in a rodent model. In addition, the facility will maintain breeding colonies of NOD SCID mice, B2MnullNOD SCID mice, and other strains that may prove to be useful in in vivo studies of immunodeficiency viruses. In summary, the Biohazard Containment Core facility will economize personnel time, eliminate duplication of effort by investigators, enhance the research capabilities of the participating investigators, and function as a central site where collaborative interactions amongst CFAR investigators is fostered/facilitated.

UMass Biohazard遏制核心设施将包括 病毒学核心设施和动物生物危害设施，将运营 具有用于体外细胞培养系统和 用于体内动物系统以支持参与的CFAR 调查人员。 病毒学核心设施将提供良好的股票 灵长类动物免疫缺陷病毒的原型实验室菌株。在 此外，该设施将为特征良好的患者提供库存 从传染性分子克隆获得的病毒的分离株和库存。 单核细胞衍生的主要CD4 T细胞中产生的病毒量 巨噬细胞或在T细胞系中将被替代和表型 特征在于合成诱导特性，复制动力学和 细胞疗法特性。病毒库存的等分试样将存档 液氮以确保病毒的持续供应。病毒核心 设施还将为CFAR提供主要的CD4 T细胞和巨噬细胞 调查人员。 FACSCAN流式细胞仪和FACSORT流式细胞仪 该设施将用于分析细胞表面标记并提供 无菌分类的细胞。动物生物危害设施将提供 希望使用的调查人员的动物，技术支持和专业知识 SCID小鼠在体内模型系统中是其艾滋病研究的一部分。这 设施将维持BL2+含量，以支持安全 与致病性免疫缺陷一起工作的生物危害环境 啮齿动物模型中的病毒。此外，该设施将保持 点头SCID小鼠，B2MNULLNOD SCID小鼠的繁殖菌落和其他 可能被证明可用于免疫缺陷的体内研究的菌株 病毒。 总而言之，生物危害遏制核心设施将节省 人事时间，消除调查人员的努力重复，增强 参与研究人员的研究能力和功能 作为CFAR之间协作互动的中心网站 调查人员被培养/促进。