CORE--BIOHAZARD CONTROL

麻省大学生物危害遏制核心设施将包括 病毒学核心设施和动物生物危害设施，并将运行 具有用于体外细胞培养系统的试剂的集中来源， 用于支持参与CFAR的体内动物系统 investigators. 病毒学核心设施将提供特征良好的库存 灵长类免疫缺陷病毒的实验室原型株。在 此外，该设施将提供特征良好的患者库存， 从感染性分子克隆获得的病毒分离物和储备物。 在原代CD 4 T细胞中产生的病毒储备物，在单核细胞来源的 巨噬细胞或T细胞系中的细胞将被滴定， 其特征在于合胞体诱导性质、复制动力学和 细胞病变特性。病毒贮备液的等分试样将存档于 液氮来保证病毒的持续供应病毒学核心 该设施还将为CFAR提供初级CD 4 T细胞和巨噬细胞 investigators. FACscan流式细胞仪和FACsort流式细胞仪在 该设备将用于分析细胞表面标记， 无菌分选的细胞。动物生物危害设施将提供 动物，技术支持和专业知识，为研究人员谁希望使用 SCID小鼠体内模型系统作为其艾滋病研究的一部分。的 设施将保持BL 2+水平的容器，以支持安全 和无生物危害的工作环境， 啮齿动物模型中的病毒。此外，该设施将保持 繁殖NOD SCID小鼠、B2 MnullNOD SCID小鼠和其他小鼠的群体 可能被证明在免疫缺陷的体内研究中有用的菌株 病毒 总之，生物危害控制核心设施将节省 人员时间，消除调查人员的重复工作， 参与研究者的研究能力和功能 作为一个中心站点，CFAR之间的协作交互 调查人员得到鼓励/便利。

The UMass Biohazard Containment Core facility will be comprises of the Virology Core Facility and the Animal Biohazard facility and will operate has a centralized source of reagents for in vitro cell-culture systems and for in vivo animal systems to support the participating CFAR investigators. The Virology Core facility will provide well-characterized stocks of prototypic laboratory strains of primate immunodeficiency viruses. In addition, the facility will provide stocks of well-characterized patient isolates and stocks of viruses obtained from infectious molecular clones. The viral stocks generated in primary CD4 T cells, in monocyte-derived macrophage, or in T cell lines, will be titered and phenotypically characterized for syncytium-inducing properties, replication kinetics and cytopathic properties. Aliquots of the viral stocks will be archived in liquid nitrogen to ensure a constant supply of virus. The Virology Core facility will also supply primary CD4 T cells and macrophages for the CFAR investigators. A FACscan flow cytometer and a FACsort flow cytometer in the facility will be used to analyze cell surface markers and provide aseptically sorted cells. The Animal Biohazard facility will provide animals, technical support and expertise for investigators who wish to use SCID mouse in vivo model systems as a part of their AIDS research. The facility will maintain a BL2+ level of contain that will support a safe and biohazard free environment for work with pathogenic immunodeficient viruses in a rodent model. In addition, the facility will maintain breeding colonies of NOD SCID mice, B2MnullNOD SCID mice, and other strains that may prove to be useful in in vivo studies of immunodeficiency viruses. In summary, the Biohazard Containment Core facility will economize personnel time, eliminate duplication of effort by investigators, enhance the research capabilities of the participating investigators, and function as a central site where collaborative interactions amongst CFAR investigators is fostered/facilitated.