CORE--BIOHAZARD CONTROL

美国大规模生物危害控制核心设施将包括 病毒学核心设施和动物生物危害设施，并将运作 拥有用于体外细胞培养系统的集中试剂来源和 用于体内动物系统支持参与的CFAR 调查人员。 病毒学核心设施将提供具有良好特征的库存 灵长类免疫缺陷病毒的实验室原型株。在……里面 此外，该设施将提供具有良好特征的患者库存 从感染性分子克隆中获得的病毒的分离物和存放物。 在原代CD4T细胞中产生的病毒储备，在单核细胞来源的 巨噬细胞，或在T细胞系中，将被滴定和表型 以合胞体诱导特性、复制动力学和 细胞病变特性。一等分的病毒库存将被存档在 液态氮，以确保病毒的持续供应。病毒学核心 该设施还将为CFAR提供原代CD4T细胞和巨噬细胞 调查人员。一台FASCAN流式细胞仪和一台FASCYPE流式细胞仪 该设施将用于分析细胞表面标记并提供 无菌分选的细胞。动物生物危害设施将提供 动物、技术支持和专业知识，为希望使用 SCID小鼠体内模型系统作为其艾滋病研究的一部分。这个 设施将维持BL2级别的容器，以支持保险箱 为病原性免疫缺陷患者提供无生物危害的工作环境 啮齿动物模型中的病毒。此外，该设施将维护 NOD SCID小鼠、B2MnullNOD SCID小鼠等繁育群体 可能被证明对免疫缺陷的体内研究有用的菌株 病毒。 总而言之，生物危害控制核心设施将节省 人员时间，消除调查人员的重复劳动，提高 参与调查人员的研究能力和职能 作为中心站点，CFAR之间的协作交互 培养/促进了调查人员。

The UMass Biohazard Containment Core facility will be comprises of the Virology Core Facility and the Animal Biohazard facility and will operate has a centralized source of reagents for in vitro cell-culture systems and for in vivo animal systems to support the participating CFAR investigators. The Virology Core facility will provide well-characterized stocks of prototypic laboratory strains of primate immunodeficiency viruses. In addition, the facility will provide stocks of well-characterized patient isolates and stocks of viruses obtained from infectious molecular clones. The viral stocks generated in primary CD4 T cells, in monocyte-derived macrophage, or in T cell lines, will be titered and phenotypically characterized for syncytium-inducing properties, replication kinetics and cytopathic properties. Aliquots of the viral stocks will be archived in liquid nitrogen to ensure a constant supply of virus. The Virology Core facility will also supply primary CD4 T cells and macrophages for the CFAR investigators. A FACscan flow cytometer and a FACsort flow cytometer in the facility will be used to analyze cell surface markers and provide aseptically sorted cells. The Animal Biohazard facility will provide animals, technical support and expertise for investigators who wish to use SCID mouse in vivo model systems as a part of their AIDS research. The facility will maintain a BL2+ level of contain that will support a safe and biohazard free environment for work with pathogenic immunodeficient viruses in a rodent model. In addition, the facility will maintain breeding colonies of NOD SCID mice, B2MnullNOD SCID mice, and other strains that may prove to be useful in in vivo studies of immunodeficiency viruses. In summary, the Biohazard Containment Core facility will economize personnel time, eliminate duplication of effort by investigators, enhance the research capabilities of the participating investigators, and function as a central site where collaborative interactions amongst CFAR investigators is fostered/facilitated.