Molecular Virology Core

分子病毒学核心

• 批准号: 8440952
• 负责人: MOHAN SOMASUNDARAN
• 金额: $ 18.21万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8440952
• 关键词: Acquired Immunodeficiency Syndrome; Biohazardous Substance; Biological Assay; Cell Line; Cells; Certification; Clinical; Collaborations; Commit; Containment; Core Facility; DNA; Detection; Diagnostic; Emerging Communicable Diseases; Ensure; Expenditure; Fostering; Funding; Grant; Guidelines; HIV-1; Human Resources; Location; Mission; Molecular; Molecular Biology; Molecular Virology; Monitor; Pathogenesis; Primate Lentiviruses; RNA; Reagent; Research; Research Personnel; Research Technics; Resources; Safety; Services; Site; Source; Students; T-Lymphocyte; Time; Titrations; Training; Tropism; United States National Institutes of Health; Viral; Viral Genome; Virus; Work; Workplace; base; cost effective; macrophage; operation; outreach; repository; web site

The overall objectives of UMass CFAR Molecular Virology Core facility are: (1) to maintain and continuously monitor the safety operations of a BSL3- and a BSL2-containment facility that will support a safe and biohazard-free environment for work with pathogenic primate immunodeficiency viruses; (2) to provide resources and services to participating CFAR investigators for their ongoing virus-related projects; and (3) to function as a central site where collaborations amongst UMass CFAR investigators is fostered. The Core services include: (1) assays for isolation, titration, phenotypic and genotypic characterization of virus; (2) providing a centralized source and repository for primary T-cells, macrophages & T-cell lines; and (3) molecular detection/diagnostic assays including qualitative and quantitative RNA and DNA-PCR for viral genomes & state of the art, cutting edge LightCycler-based PCR assays for real-time detection and quantitation of HIV-1. The Molecular Virology Core facility, in conjunction with UMass CFAR-Clinical and Molecular Biology Core facilities, uphold the mission of UMass CFAR by providing standardized and centralized routine research techniques and reagents; reducing expenditure by eliminating duplication of effort & by efficient use of personnel; and fostering new collaborative projects and grants. The Molecular Virology Core is also committed to provide educational & training opportunities to CFAR investigators and students/fellows in working within BSL3 containment facility, in developing new molecular virology assays, in BSL3 Certification, and in PCR Certification. Details of the Molecular Virology Core facility, its location, its functions, the services provided are all available on the UMass CFAR Molecular Virology Core website (www.umassmed.edu/cfar/viroloavcore).

麻省理工大学CFAR分子病毒学核心设施的总体目标是：(1)维持和持续监测BSL3和bsl2遏制设施的安全运行，这将为致病性灵长类免疫缺陷病毒的工作提供安全和无生物危害的环境；(2)为参与的CFAR调查人员正在进行的病毒相关项目提供资源和服务；(3)作为促进马萨诸塞大学CFAR研究人员之间合作的中心站点。核心服务包括：(1)病毒的分离、滴定、表型和基因型鉴定；(2)为原代t细胞、巨噬细胞和t细胞系提供集中的来源和存储库；(3)分子检测/诊断分析，包括用于病毒基因组的定性和定量RNA和DNA-PCR和最新技术，用于实时检测和定量HIV-1的基于lightcycler的尖端PCR分析。分子病毒学核心设施与麻省理工学院临床和分子生物学核心设施一起，通过提供标准化和集中的常规研究技术和试剂，维护麻省理工学院临床和分子生物学核心设施的使命；通过消除重复来减少开支