Molecular Virology Core

分子病毒学核心

• 批准号: 7927081
• 负责人: MOHAN SOMASUNDARAN
• 金额: $ 24.96万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7927081
• 关键词: Acquired Immunodeficiency Syndrome; Arts; Biohazardous Substance; Biological Assay; Cell Line; Cells; Certification; Clinical; Collaborations; Commit; Containment; Core Facility; Detection; Diagnostic; Emerging Communicable Diseases; Ensure; Expenditure; Fostering; Funding; Grant; Guidelines; HIV-1; Human Resources; Location; Mission; Molecular; Molecular Biology; Molecular Virology; Monitor; Operative Surgical Procedures; Pathogenesis; Primate Lentiviruses; RNA; Reagent; Research; Research Personnel; Research Technics; Resources; Safety; Services; Site; Source; Students; T-Lymphocyte; Time; Titrations; Training; Tropism; United States National Institutes of Health; Viral; Viral Genome; Virus; Work; Workplace; base; macrophage; outreach; repository; web site

The overall objectives of UMass CFAR Molecular Virology Core facility are: (1) to maintain and continuously monitor the safety operations of a BSL3- and a BSL2-containment facility that will support a safe and biohazard-free environment for work with pathogenic primate immunodeficiency viruses; (2) to provide resources and services to participating CFAR investigators for their ongoing virus-related projects; and (3) to function as a central site where collaborations amongst UMass CFAR investigators is fostered. The Core services include: (1) assays for isolation, titration, phenotypic and genotypic characterization of virus; (2) providing a centralized source and repository for primary T-cells, macrophages & T-cell lines; and (3) molecular detection/diagnostic assays including qualitative and quantitative RNA and DNA-PCR for viral genomes & state of the art, cutting edge LightCycler-based PCR assays for real-time detection and quantitation of HIV-1. The Molecular Virology Core facility, in conjunction with UMass CFAR-Clinical and Molecular Biology Core facilities, uphold the mission of UMass CFAR by providing standardized and centralized routine research techniques and reagents; reducing expenditure by eliminating duplication of effort & by efficient use of personnel; and fostering new collaborative projects and grants. The Molecular Virology Core is also committed to provide educational & training opportunities to CFAR investigators and students/fellows in working within BSL3 containment facility, in developing new molecular virology assays, in BSL3 Certification, and in PCR Certification. Details of the Molecular Virology Core facility, its location, its functions, the services provided are all available on the UMass CFAR Molecular Virology Core website (www.umassmed.edu/cfar/viroloavcore).

UMassCFAR分子病毒学核心设施的总体目标是：(1)维护和持续 监控BSL3-和BSL2-安全壳设施的安全操作，该设施将支持安全和 与致病性灵长类免疫缺陷病毒一起工作的无生物危害环境；(2)提供 为参与的CFAR调查员正在进行的病毒相关项目提供资源和服务；以及 作为一个中心站点，促进了UMassCFAR调查人员之间的合作。《核心》 服务包括：(1)病毒的分离、滴定、表型和基因表型鉴定；(2) 为原代T细胞、巨噬细胞和T细胞系提供集中的来源和储存库；以及(3) 病毒的分子检测/诊断分析，包括定性和定量RNA和DNA-PCR 基因组&先进的、基于LightCycler的实时检测和 HIV-1的定量检测。分子病毒学核心设施，与UMassCFAR-临床和 分子生物学核心设施，通过提供标准化和 集中常规研究技术和试剂；通过消除重复的 努力&通过有效利用人员；以及促进新的合作项目和赠款。《分子》 病毒学核心还致力于为CFAR调查人员和 学生/研究员在BSL3安全壳设施内工作，开发新的分子病毒学测试，在 BSL3认证，以及在PCR认证中。分子病毒学核心设施的详细信息，其位置，其 功能、所提供的服务均可在UMassCFAR分子病毒学核心网站上获得 (www.umassmed.edu/cfar/viroloavcore)。