CORE--MOLECULAR BIOLOGY FACILITY

达纳-法伯癌症研究所建立了一个分子生物学核心 帮助研究所实验室更轻松、更具成本效益的设施 使用分子生物学技术和试剂，如寡核苷酸， 合成肽、微蛋白测序、质量分析、DNA 核酸测序、蛋白质配基分析和电子显微镜 酸和核酸-蛋白质的相互作用。此应用程序请求 用于支付部分费用的资金，这些费用不属于按存储容量使用计费系统。这个 设施包括以下主要设备：应用生物系统394 DNA/RNA合成仪，应用生物系统380B DNA合成仪，已应用 生物系统477蛋白质测序仪，120A分析仪，应用生物系统430 多肽合成器，惠普HP1090微孔液 2 Waters高效液相色谱仪的应用生物系统420/H-130氨基酸 芬兰激光飞行时间质谱仪，Pharmacia Biacore 生物传感器，应用生物系统373 DNA测序仪，蔡司EM 10A电子 显微镜。该设施由一名设施主管和4名员工组成。 提供不同服务的时间技术人员以及提供的 对希望使用特定工具的调查人员的说明 他们自己。

The Dana-Farber Cancer Institute has established a Molecular Biology Core Facility to help Institute laboratories more easily and cost effectively use molecular biology techniques and reagents such as oligonucleotides, synthetic peptides, microprotein sequencing, mass analysis, DNA sequencing, protein-ligand assays and electron microscopy of nucleic acids and nucleic acid-protein interactions. This application requests funds to partially cover costs not covered by a charge back system. The facility contains the following major equipment: Applied Biosystems 394 DNA/RNA Synthesizer, Applied Biosystems 380B DNA Synthesizer, Applied Biosystems 477 Protein Sequencer, 120A Analyzer, Applied Biosystems 430 Peptide Synthesizer, Hewlett Packard HP1090 Micropore Liquid Chromatograph, 2 Waters HPLC's Applied Biosystems 420/H-130 Amino Acid Analyzer, Finnigan Lasermat TOF Mass Analyzer, Pharmacia BIAcore biosensor, Applied Biosystems 373 DNA Sequencer, Zeiss EM 10A Electron Microscope. The facility is staffed by a facility director and 4 full time technicians who provide the different services as well as provided instruction to investigators who wish to use specific instruments themselves.