CANDIDA VIRULENCE AND HUMAN AGING

念珠菌毒力与人类衰老

• 批准号: 6104873
• 负责人: DAVID R. SOLL
• 金额: $ 13.44万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6104873
• 关键词: Candida albicans; age difference; aging; antifungal agents; biochemical evolution; candidiasis; cell adhesion; computer assisted sequence analysis; dentures; epithelium; fungal genetics; gene expression; genetic strain; human subject; laboratory mouse; microorganism culture; molecular cloning; nucleic acid probes; oral mucosa; phenotype; restriction fragment length polymorphism; southern blotting; stomatitis; symbiosis; virulence

Candida albicans is a pathogenic yeast which resides as a commensal in the oral cavity of a majority of healthy individuals. However, under a number of predisposing conditions, C. albicans invades tissue causing infection. It has been assumed that commensal strains are the source of subsequent infection and that there is little strain specialization. However, these assumptions have recently been questioned by results which demonstrate that different strains are carried in different anatomical locations of the same healthy women, that strain replacement occurs in the oral cavity of AIDS patients and male partners of vaginitis patients, that each strain has a number of general phenotypes, regulated by a reversible, high frequency switching mechanism, which may be utilized under different environmental conditions, and that stains can rapidly evolve at the genetic level. We, therefore, will test the genetic relationship between commensal and infecting strains of the oral cavity of the same individual in the transition from a healthy state to denture stomatitis. For this study, we have developed a computer-assisted DNA fingerprinting system employing the moderately repetitive probe Ca3, and conditions for analyzing phenotype switching. In addition, we will compare commensal and infecting strains from the same individuals for a number of putative virulence traits. This study will answer at the generic level 1) whether phenotypic switching is involved in the transmission from commensalism to pathogenesis, and 4) whether commensal and infecting strains differ in phenotype.

白色念珠菌是一种致病性酵母菌， 大多数健康人的口腔。 然而，在A 诱发条件的数量，C.白色念珠菌侵入组织， 感染 据推测，真菌菌株是 随后的感染，几乎没有菌株特化。 然而，这些假设最近受到了一些结果的质疑， 证明不同的菌株在不同的解剖结构中携带， 同样的健康女性的位置，应变替代发生在 艾滋病患者的口腔和阴道炎患者的男性伴侣， 每种菌株都有许多一般表型，由一种 可逆的高频开关机构，其可以被利用 在不同的环境条件下， 在基因层面上进化。 因此，我们将测试 口腔细菌与感染菌株的关系 从健康状态到假牙的过渡 口腔炎 为了这项研究，我们开发了一种计算机辅助DNA 使用适度重复探针Ca3的指纹识别系统，和 分析表型转换的条件。 此外，我们将 比较来自同一个体的寄生虫和感染菌株， 推定的毒力性状的数量。 这项研究将在 基因水平1）表型转换是否参与了 从过敏症到发病的传播，以及4）是否过敏 并且感染菌株在表型上不同。