CHEMOTAXIS AND CHEMOKINESIS IN DICTYOSTELIUM

拟议实验的目标是继续 使用复杂的两个 三维和三维计算机辅助运动分析 系统. Soll博士将继续研究 肌动蛋白相关蛋白在细胞运动过程中的作用 这种生物的网柄藻和细胞激酶突变体。 一个主要 目的是完成严格的三维动态分析的细胞 包膜、细胞核、细胞质颗粒和颗粒- 细胞分裂期间伪足的非颗粒界面 易位 本部分的研究将利用新的 开发了高分辨率3D-DIAS重建软件， 包括开发的直接图像重建软件包 在核心设施。

The goal of the proposed experiments is to continue the characterization of Dictyostelium motility using sophisticated two dimensional and three dimensional computer-assisted motion analysis systems. Dr. Soll will continue to investigate the functional roles of actin-associated proteins in the process of cell motility using Dictyostelium and cytoakeletal mutants of this organism. A major objective is to complete a rigorous 3D dynamic analysis of the cell envelope, the nucleus, cytoplasmic particles and the particulate- nonparticulate interphase of the pseudopod during cellular translocation. This part of the study will make use of the newly developed high resolution 3D-DIAS reconstruction software which includes the direct image reconstruction software package developed in the Core Facility.