CHEMOTAXIS AND CHEMOKINESIS IN DICTYOSTELIUM

拟议中的实验的目标是继续 用复杂的两种方法研究网柄网柄藻的运动性 三维和三维计算机辅助运动分析 系统。索尔博士将继续研究 肌动蛋白相关蛋白在细胞运动过程中的作用 这种生物的网柄和细胞骨架突变体。一位少校 目标是完成对细胞的严格3D动态分析 包膜、细胞核、细胞质颗粒和颗粒- 伪足细胞过程中的非颗粒间期 易位。这部分研究将利用新的 开发了高分辨率3D-DIAS重建软件 包括开发的直接图像重建软件包 在核心设施中。

The goal of the proposed experiments is to continue the characterization of Dictyostelium motility using sophisticated two dimensional and three dimensional computer-assisted motion analysis systems. Dr. Soll will continue to investigate the functional roles of actin-associated proteins in the process of cell motility using Dictyostelium and cytoakeletal mutants of this organism. A major objective is to complete a rigorous 3D dynamic analysis of the cell envelope, the nucleus, cytoplasmic particles and the particulate- nonparticulate interphase of the pseudopod during cellular translocation. This part of the study will make use of the newly developed high resolution 3D-DIAS reconstruction software which includes the direct image reconstruction software package developed in the Core Facility.