CHEMOTAXIS AND CHEMOKINESIS IN DICTYOSTELIUM

盘基网柄菌的趋化性和趋化作用

• 批准号: 6108403
• 负责人: DAVID R. SOLL
• 金额: $ 16.82万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6108403
• 关键词: Dictyostelium; actin binding protein; cell biology; cell migration; chemoattractants; chemokine; chemotaxis; cyclic AMP; cytoskeletal proteins; image processing; immunofluorescence technique; microorganism culture; mutant; myosin light chain kinase; myosins; nonmammalian vertebrate embryology

The goal of the proposed experiments is to continue the characterization of Dictyostelium motility using sophisticated two dimensional and three dimensional computer-assisted motion analysis systems. Dr. Soll will continue to investigate the functional roles of actin-associated proteins in the process of cell motility using Dictyostelium and cytoakeletal mutants of this organism. A major objective is to complete a rigorous 3D dynamic analysis of the cell envelope, the nucleus, cytoplasmic particles and the particulate- nonparticulate interphase of the pseudopod during cellular translocation. This part of the study will make use of the newly developed high resolution 3D-DIAS reconstruction software which includes the direct image reconstruction software package developed in the Core Facility.

拟议实验的目标是继续 使用复杂的两种方法表征盘基网柄菌的运动性 三维和三维计算机辅助运动分析 系统. Soll博士将继续研究 肌动蛋白相关蛋白在细胞运动过程中的作用 这种生物的网柄藻和细胞激酶突变体。 一个主要 目的是完成严格的三维动态分析的细胞 包膜、细胞核、细胞质颗粒和颗粒- 细胞分裂期间伪足的非颗粒界面 易位 本部分的研究将利用新的 开发了高分辨率3D-DIAS重建软件， 包括开发的直接图像重建软件包 在核心设施。