CHEMOTAXIS AND CHEMOKINESIS IN DICTYOSTELIUM

盘基网柄菌的趋化性和趋化作用

• 批准号: 6272072
• 负责人: DAVID R. SOLL
• 金额: $ 16.34万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6272072
• 关键词: Dictyostelium; actin binding protein; cell biology; cell migration; chemoattractants; chemokine; chemotaxis; cyclic AMP; cytoskeletal proteins; image processing; immunofluorescence technique; microorganism culture; mutant; myosin light chain kinase; myosins; nonmammalian vertebrate embryology

The goal of the proposed experiments is to continue the characterization of Dictyostelium motility using sophisticated two dimensional and three dimensional computer-assisted motion analysis systems. Dr. Soll will continue to investigate the functional roles of actin-associated proteins in the process of cell motility using Dictyostelium and cytoakeletal mutants of this organism. A major objective is to complete a rigorous 3D dynamic analysis of the cell envelope, the nucleus, cytoplasmic particles and the particulate- nonparticulate interphase of the pseudopod during cellular translocation. This part of the study will make use of the newly developed high resolution 3D-DIAS reconstruction software which includes the direct image reconstruction software package developed in the Core Facility.

所提出的实验的目标是继续