PILOT--SYSTEM FOR SIMULTANEOUS OPTICAL AND PATCH CLAMP RECORDING FROM HEPATOCYTES

我们建议开发一种新的基于计算机的系统来执行 单细胞同时视频成像和膜片钳记录 还有对联。尽管存在针对以下各项功能的系统 荧光成像、膜片钳电流记录和电容 测量，这些测量的同步和集成 在一个实验系统中还没有完成。我们建议 分三个阶段开发这样一个系统。在这份提案中，我们要求 为前两个阶段提供资金，这两个阶段涉及设计和 实施控制和同步数据的软件 收集和分析实验数据： 1.软件设计和膜片钳接口。在第一年，我们 打算规划软件的体系结构，并根据现有情况进行调整 基于Macintosh的膜片钳采集程序 QuickTime同步系统。 2.图像定量分析的实现。在第二种情况下工作 年将是执行荧光比率的模块的改编 和形态图像处理，以在QuickTime框架中工作。 这些软件开发构成了第三阶段的基础， 搭建了实际的实验装置。

We propose to develop a novel computer-based system for performing simultaneous video imaging and patch-clamp recording from single cells and couplets. Although systems exist for the individual functions of fluorescence imaging, patch-clamp current recording and capacitance measurement, the synchronization and integration of these measurements in one experimental system has not been accomplished. We propose to develop such a system in three stages. In this proposal we request funding for the first two stages, which involve the design and implementation of the software that will control and synchronize the data collection and analysis for experiments: 1. Software design and patch-clamp interface. In the first year we intend to plan the architecture of the software, and to adapt as existing Macintosh-based patch-clamp acquisition program to work with the QuickTime synchronization system. 2. Implementation of quantitative image analysis. Work in the second year will be the adaptation of modules that perform fluorescence ratio and morphometric image processing to work in the QuickTime framework. These software developments form the basis for the third stage, the construction of the actual experimental setup.