Patch Clamp Amplifiers on a Chip

描述（由申请人提供）：膜片钳技术是表征离子通道功能的主要工具。对于功能基因组学和药物发现，迫切需要能够执行高通量（HTS）膜片钳记录。我们建议开发一种使能技术的高温超导记录集成到一个单一的单片集成电路的高性能膜片钳放大器。我们的目标是开发一个包含多个放大器的芯片，并开发相关的接口电子产品。我们的芯片的高密度组装将允许多达384个自动膜片钳放大器与相应的平面膜片钳电极阵列相匹配，从而允许并行膜片钳测量。公共卫生相关性：相关性离子通道的活性负责非常多的生理功能和许多病理生理学的基础，从癫痫和心律失常到囊性纤维化和耳聋。离子通道功能的表征以及改变该功能的药物的开发迫切需要允许“高通量”测量的新仪器。

DESCRIPTION (provided by applicant): The patch-clamp technique is the central tool for characterizing ion channel function. For functional genomics and for drug discovery, the ability to perform high-throughput (HTS) patch clamp recordings is urgently needed. We propose to develop an enabling technology for HTS recordings by integrating a high-performance patch-clamp amplifier into a single monolithic integrated circuit. Our goal is to develop a chip containing multiple amplifiers and to develop the associated interfacing electronics. High-density assembly of our chips will allow as many as 384 automated patch-amplifiers to be matched to a corresponding array of planar patch-clamp electrodes allowing massively-parallel patch-clamp measurements. PUBLIC HEALTH RELEVANCE: Relevance The activity of ion channels is responsible for very many physiological functions and underlie many pathophysiologies, ranging from epilepsy and cardiac arrhythmias to Cystic Fibrosis and deafness. The characterization of ion channel function, and the development of drugs that modify this function, urgently requires new instrumentation that allows "high throughput" measurements.