Patch Clamp Amplifiers on a Chip

描述（由申请人提供）：膜片钳技术是表征离子通道功能的核心工具。对于功能基因组学和药物发现，迫切需要进行高通量（HTS）膜片钳记录的能力。我们建议通过将高性能膜片钳放大器集成到单个单片集成电路中来开发一种使能HTS记录的技术。我们的目标是开发一个包含多个放大器的芯片，并开发相关的接口电子器件。我们芯片的高密度组装将允许多达384个自动化贴片放大器与相应的平面贴片钳电极阵列相匹配，从而实现大规模平行的贴片钳测量。离子通道的活性负责许多生理功能，并且是许多病理生理学的基础，从癫痫和心律失常到囊性纤维化和耳聋。离子通道功能的表征，以及改变这种功能的药物的开发，迫切需要允许“高通量”测量的新仪器。

DESCRIPTION (provided by applicant): The patch-clamp technique is the central tool for characterizing ion channel function. For functional genomics and for drug discovery, the ability to perform high-throughput (HTS) patch clamp recordings is urgently needed. We propose to develop an enabling technology for HTS recordings by integrating a high-performance patch-clamp amplifier into a single monolithic integrated circuit. Our goal is to develop a chip containing multiple amplifiers and to develop the associated interfacing electronics. High-density assembly of our chips will allow as many as 384 automated patch-amplifiers to be matched to a corresponding array of planar patch-clamp electrodes allowing massively-parallel patch-clamp measurements. PUBLIC HEALTH RELEVANCE: Relevance The activity of ion channels is responsible for very many physiological functions and underlie many pathophysiologies, ranging from epilepsy and cardiac arrhythmias to Cystic Fibrosis and deafness. The characterization of ion channel function, and the development of drugs that modify this function, urgently requires new instrumentation that allows "high throughput" measurements.