FIELD-EMISSION ELECTRON MICROSCOPE FOR MACROMOLECULES

大分子场发射电子显微镜

• 批准号: 6052309
• 负责人: FREDERICK J SIGWORTH
• 金额: $ 50万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6052309
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; cryoelectron microscopy; transmission electron microscopy

The purpose of the proposed grant is to assist in the purchase of a state- of-the-art, field emission electron microscope for the study of macromolecular structures. This microscope is to be the centerpiece of a new initiative in structural biology at Yale School of Medicine. Yale has a distinguished history in the determination of macromolecular structures by X-ray diffraction, and also a distinguished history in the use of electron microscopy in cell biology. Until now there have been no facilities for cryo-electronmicroscopy of macromolecular assemblies, an approach that bridges the two areas. We propose the purchase of a Philips Tecnai F20 transmission electron microscope with accessories for lowdose imaging of cryogenically cooled specimens. This field-emission microscope will be used for structure determination of membrane proteins in two-dimensional crystals, and for three-dimensional reconstruction of macromolecular structures using single-particle techniques.

建议拨款的目的，是资助研究所购置一部先进的场发射电子显微镜，以研究高分子结构。这台显微镜将成为耶鲁医学院结构生物学新项目的核心。耶鲁大学在通过X射线衍射测定大分子结构方面有着杰出的历史，在细胞生物学中使用电子显微镜方面也有着杰出的历史。到目前为止，还没有大分子组装的低温电子显微镜设备，这是一种连接这两个领域的方法。我们建议购买一台Philips Tecnai F20透射电子显微镜及其配件，用于低温冷却标本的低剂量成像。这种场发射显微镜将用于二维晶体中膜蛋白的结构测定，以及使用单粒子技术进行大分子结构的三维重建。

项目成果

Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy.

• DOI: 10.1038/nature08291
• 发表时间: 2009-09-10
• 期刊: Nature
• 影响因子: 64.8

Two conformational states of the membrane-associated Bacillus thuringiensis Cry4Ba delta-endotoxin complex revealed by electron crystallography: implications for toxin-pore formation.

电子晶体学揭示的膜相关苏云金芽孢杆菌 Cry4Ba δ-内毒素复合物的两种构象状态：对毒素孔形成的影响。

• DOI: 10.1016/j.bbrc.2007.07.086
• 发表时间: 2007
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Ounjai,Puey;Unger,VinzenzM;Sigworth,FredJ;Angsuthanasombat,Chanan
• 通讯作者: Angsuthanasombat,Chanan