SENSORY PROCESSING BY SUBFORNICAL ORGAN IN BODY FLUID/CARDIOVASCULAR HOMEOSTASIS

体液/心血管稳态中隐窝下器官的感觉处理

• 批准号: 6272498
• 负责人: ALAN Kim JOHNSON
• 金额: $ 18.15万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-06 至 1998-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6272498
• 关键词: angiotensin II; blood brain barrier; blood volume; body fluids; chemoreceptors; electrolyte balance; electrophysiology; hemodynamics; homeostasis; hormone receptor; hormone regulation /control mechanism; hypertension; laboratory rat; neural information processing; neuroanatomy; neuroregulation; saluresis; sensory receptors; sympathetic nervous system; telencephalon; vasodilation; water drinking behavior

Body fluid and cardiovascular homeostasis depends on ingestive behaviors and on reflexes acting to modulate renal water and sodium loss and to redistribute blood flow. In order for the appropriate effector mechanisms that maintain body fluid balance and cardiovascular function to be engaged, the brain must receive information about the current status of body hydration and blood pressure. Several visceral sensory systems provide this type of information to the central nervous system (CNS). This input is carried over afferent neural pathways and by the direct action of circulating factors on select brain regions which lack a blood-brain barrier. Three permeable areas of the brain receiving blood-borne signals have been described as sensory circumventricular organs (CVOs) and are typified by the subfornical organ (SFO). This proposal focuses on the SFO and its role in detecting circulating angiotensin II, how this information is handled within this structure and ultimately transferred into forebrain regions involved in the behavioral and reflex control of hydromineral balance and blood pressure. The present proposal builds upon our prior studies of the central sensing and processing of afferent signals involved in body fluid and cardiovascular homeostasis. The aims of this research are to investigate the mechanisms of sensory transfer and neural processing with the SFO in response to blood-borne ANG II and then to characterize the pathways which convey this information to specific forebrain nuclei involved in maintaining cardiovascular and body fluid homeostasis. The studies will employ both in vivo and in vitro methods to electrophysiologically, pharmacologically and anatomically characterize identified cells with the SFO. The cells will be analyzed to understand the mechanisms they use for both intra- and inter- cellular signaling. New information derived from studies on this important sensory structure that couples the systemic circulation with the CNS proper has relevance for understanding both normal physiology and pathophysiology associated with states of disordered body fluid and cardiovascular regulation, such as in hypertension.

体液和心血管稳态取决于摄入行为 以及调节肾水和钠损失的反射 重新分配血流。为了适当的效应机制 维持体液平衡和心血管功能 参与时，大脑必须接收有关当前状态的信息 身体水分和血压。几种内脏感觉系统 向中枢神经系统（CNS）提供此类信息。这 输入通过传入神经通路进行，并通过直接作用 缺乏血脑的特定大脑区域的循环因子 障碍。大脑的三个可渗透区域接收血液信号 被描述为感觉室周器官（CVO）并且是 以穹窿下器官（SFO）为代表。 该提案重点关注 SFO 及其在检测流通中的作用 血管紧张素 II，如何在该结构中处理该信息以及 最终转移到参与行为的前脑区域 以及水矿物质平衡和血压的反射控制。现在的 该提案建立在我们之前对中央传感和 涉及体液和心血管的传入信号的处理 体内平衡。本研究的目的是调查其机制 感觉传递和神经处理与 SFO 的响应 血源性 ANG II，然后表征传达此信息的途径 信息传递给参与维持的特定前脑核 心血管和体液稳态。这些研究将同时采用 体内和体外方法，以电生理学、药理学和 使用 SFO 对已识别的细胞进行解剖学表征。细胞将是 分析以了解他们用于内部和外部的机制 细胞信号传导。来自这一重要研究的新信息 连接体循环与中枢神经系统的感觉结构 正确地与理解正常生理学和 与体液紊乱状态相关的病理生理学 心血管调节，例如高血压。