EFFECT OF CYTOKINES IN HOST DEFENSE AND INFLAMMATION

细胞因子在宿主防御和炎症中的作用

• 批准号: 6098953
• 负责人: John I Gallin
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6098953
• 关键词: adolescence (12-20); antiinflammatory agents; bactericidal immunity; cellular immunity; chemoattractants; child (0-11); clinical research; cyclosporines; cytokine; fibrinogen; human subject; inflammation; interleukin 8; microorganism immunology; neutrophil; protein biosynthesis

Accumulation of neutrophils to sites of infection and inflammation is essential to host defense. When too few neutrophils accumulate there is increased susceptibility to infection. When too many neutrophils accumulate there can be tissue destruction. In recent years cytokines, products of numerous types of cells, have been shown to attract neutrophils to inflammation. One of these cytokines, IL-8 is critical for mobilizing normal numbers of neutrophils to inflammatory sites. This past year our investigations of the synthesis of IL-8, a potent chemoattractant, by neutrophils were continued. Previously we demonstrated that when human blood neutrophil s migrate into tissues they are induced to make abundant amounts of IL-8. In fiscal year '97 we utilized peripheral blood normal neutrophils from human subjects and we studied calcium metabolism and IL-8 synthesis and release. Our studies demonstrated two pathways for neutrophil IL-8 synthesis, one calcium dependent and the other calcium independent.

中性粒细胞在感染和炎症部位积聚 对主机防御至关重要。 当中性粒细胞积累太少时 是感染的易感性增加。 当中性粒细胞过多时 积累可能会造成组织破坏。 最近几年 细胞因子是多种细胞的产物，已被证明可以 吸引中性粒细胞产生炎症。 IL-8 是这些细胞因子之一 对于动员正常数量的中性粒细胞产生炎症至关重要 网站。去年我们对 IL-8 的合成进行了研究，IL-8 是一种 中性粒细胞继续产生有效的化学引诱剂。 以前我们 证明当人类血液中性粒细胞迁移到组织中时 它们被诱导产生大量的 IL-8。 97财年 我们利用人类受试者的外周血正常中性粒细胞， 我们研究了钙代谢和IL-8的合成和释放。 我们的 研究表明中性粒细胞 IL-8 合成有两种途径，一种是 钙依赖性，另一种钙非依赖性。