Developmental Immunotherapeutics for Allergic Diseases and Asthma

过敏性疾病和哮喘的发育免疫治疗

基本信息

项目摘要

This project seeks to develop novel immunologic therapies for allergic diseases as well as the Laboratory techniques required to understand their mechanism of action and rational application. We have developed a highly sensitive allergen specific T cell cytokine assay which allows us a direct high through put means to examine cytokine responses in clinical trials of allergen immunotherapy. This technique directly measures both the frequency and cytokine profile of antigen specific T cells and provides insight into the mechanism of action of immunomodulatory therapies. Strategies that are being pursued include the administration of rhIL-12 as an adjuvant in allergen immunotherapy as well as studies of the mechanisms of action of traditional immunotherapy. We are applying a similar approach to mouse vaccine models to understand how DNA vaccines generate specific immune responses. To date, we have developed an antigen specific assay utilizing cytometry, allowing the direct detection of complex cytokine phenotypes at the single cell level. We have examined the cytokine response to aeroallergens such as house dust, mite and cat, demonstrating a uniform Th2 response in allergic asthmatics but little or not detectable response in healthy controls. These results suggest that the normal T cell response to ubiquitous aeroallergens is tolerance. A phase I trial using rhIL-12 as an adjuvant in allergen immunotherapy has been approved by the NIAID, IRB and is waiting FDA approval. Antigen specific cytokine flow has been applied to DNA vaccination of mice, demonstrating that DNA vaccination creates a long lasting Th1 polarized immune response in both CD4+ and CD8+ subsets, whereas protein immunization generates a more transient response limited to CD4 T cells.
本项目旨在开发新型免疫制剂

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Dean D Metcalfe其他文献

Plasma IL-6 correlates with disease category and with hematological parameters in patients with mastocytosis
  • DOI:
    10.1016/s0091-6749(02)81601-3
  • 发表时间:
    2002-01-01
  • 期刊:
  • 影响因子:
  • 作者:
    Knut Brockow;Cem Akin;Mary M Huber;Dean D Metcalfe
  • 通讯作者:
    Dean D Metcalfe
Comparison of FceRI and FcγRI-dependent signaling pathways in human mast cells
  • DOI:
    10.1016/s0091-6749(02)82259-x
  • 发表时间:
    2002-01-01
  • 期刊:
  • 影响因子:
  • 作者:
    Christine Tkaczyk;Yoshimishi Okayama;Dean D Metcalfe;Alasdair M Gilfillan
  • 通讯作者:
    Alasdair M Gilfillan
Serum tryptase levels combined with flow cytometric analysis of bone marrow aspirate mast cells differentiate systemic mastocytosis from idiopathic syndromes
  • DOI:
    10.1016/s0091-6749(02)81675-x
  • 发表时间:
    2002-01-01
  • 期刊:
  • 影响因子:
  • 作者:
    Cem Akin;Arnold S Kirshenbaum;Dean D Metcalfe
  • 通讯作者:
    Dean D Metcalfe
Direct determination of allergen specific T cell cytokine responses during immunotherapy
  • DOI:
    10.1016/s0091-6749(02)82225-4
  • 发表时间:
    2002-01-01
  • 期刊:
  • 影响因子:
  • 作者:
    L Brigida Cayosa Hunter;Varatda Plainetr;Barbara Foster;Mary M Huber;Dean D Metcalfe;Calman Prussin
  • 通讯作者:
    Calman Prussin

Dean D Metcalfe的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Dean D Metcalfe', 18)}}的其他基金

REGULATION OF CYTOKINE GENE EXPRESSION IN MAST CELLS
肥大细胞中细胞因子基因表达的调节
  • 批准号:
    6098983
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Fc Receptors in Mast Cell Signaling and Function
肥大细胞信号传导和功能中的 Fc 受体
  • 批准号:
    6431716
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Pathogenesis of Physical Urticaria Syndromes
物理性荨麻疹综合征的发病机制
  • 批准号:
    8946474
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
The Pathogenesis, Diagnosis, And Treatment Of Systemic Mast Cell Disorders
系统性肥大细胞疾病的发病机制、诊断和治疗
  • 批准号:
    9354692
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
The Pathogenesis, Diagnosis, And Treatment Of Systemic Mast Cell Disorders
系统性肥大细胞疾病的发病机制、诊断和治疗
  • 批准号:
    10014014
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Pathogenesis and Treatment of Anaphylaxis
过敏反应的发病机制和治疗
  • 批准号:
    10014172
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
The Pathogenesis, Diagnosis, And Treatment of Systemic Mast Cell Disorders
系统性肥大细胞疾病的发病机制、诊断和治疗
  • 批准号:
    10272016
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Molecular Biology Of Mast Cell Growth And Differentiation
肥大细胞生长和分化的分子生物学
  • 批准号:
    7732464
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
The Pathogenesis, Diagnosis, And Treatment Of Systemic Mast Cell Disorders
系统性肥大细胞疾病的发病机制、诊断和治疗
  • 批准号:
    7964210
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Activation of Mast Cells in Disease States: Pharmacological Modification
疾病状态下肥大细胞的激活:药理学修饰
  • 批准号:
    7964545
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:

相似海外基金

In Vitro Dispersion Studies of Airborne Allergen Particles
空气中过敏原颗粒的体外分散研究
  • 批准号:
    564075-2021
  • 财政年份:
    2021
  • 资助金额:
    --
  • 项目类别:
    University Undergraduate Student Research Awards
AIRWAY RESPONSE TO AIRBORNE ALLERGEN
对空气中过敏原的气道反应
  • 批准号:
    3883709
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了