MECHANISMS GOVERNING SPINDLE ALIGNMENT IN C ELEGANS

在线虫胚胎的最初几次分裂中， 增殖分裂，其中两个子体是相等的， 和差异化的划分，在这些划分中， 命运 目前正在研究这些物质的动力特性。 细胞骨架是分化分裂所特有的。 在 特别是：发现因素被隔离到一个区域的 分裂前的细胞;分裂通常不对称， 有丝分裂器中心体的不对称性;有丝分裂的 可以看到装置在预先形成的极性轴上对准。 的 该项目的目的是使用体内成像和相关的 电子显微镜来研究这些现象， as to the mechanisms机制used in the generationof cell细胞diversity多样性. 的 本研究的重点是阐明微管在 定位纺锤体在早期不对称细胞分裂 秀丽隐杆线虫 我们将使用活体荧光显微镜 为了表征微管在细胞生长过程中的组织和动力学， 野生型和突变体胚胎中纺锤体排列。 我们特别 感兴趣的突变体， 微管的组织，以确定基因参与 主轴对准。 此外，我们还将研究 皮质微管附着位点。 结果将提供洞察力 深入到分裂的基本机制和分子组成 决定性细胞分裂的平面规格。 SCXENTXFXC 子项目授权编号：P41 RR 00570 -27

The first few divisions in C elegans embryo has examples of both proliferative divisions in which both the daughters are equivalent, and differentiative divisions in which the daughters take on different fates. Studies are being undertaken of the dynamic properties of the cytoskeleton that are unique to differentiative divisions. In particular: factors are found to be segregated to one region of the cell prior to division; divisions are usually asymmetrical due to an asymmetry in the centrosomes of the mitotic apparatus; the mitotic apparatus is seen to align on a preformed axis of polarity. The purpose of this project is to use both in vivo imaging and correlative electron microscopy to study these phenomenon so as to gain insights as to the mechanisms used in the generationof cell diversity. The focus of this study is to elucidate the role of microtubules in positioning the spindle in early asymmetric cell divisions of Caenorhabditis elegans. We will use in vivo fluorescence microscopy to characterize the organization and dynamics of microtubules during spindle alignment in wildtype and mutant embryos. We are especially interested in mutants that exhibit alterations in the dynamic organization of microtubules in order to identify genes involved in spindle alignment. In addition, we will examine the ultrastructure of cortical microtubule attachment sites. Results will provide insight into the basic mechanisms and molecular components underlying cleavage plane specification in determinative cell divisions. SCXENTXFXC SUBPROJECT GRANT NUMBER: P41RR00570-27