CHARACTERIZE STRUCT OF MOLECULAR CHAPERONES CYTOSTRUCTURE OF PROKARYOTIC CELL

我们正在研究一种嗜热性超强病毒的超微结构 古生菌，柴胡，假设分子 这种有机体中的伴侣蛋白形成了丝状 真核生物的原核生物版本的细胞结构 细胞骨架。目标是确认丝状菌的存在 以前在这些生物中看到的结构，并阐明它们的 组成；即建立它们与分子的关系 陪伴者。战略是尝试优化 使用快速冷冻技术的细胞质结构、HR-SEM和 能量过滤显微镜。与细丝相关的蛋白质将 通过免疫胶体金或荧光技术进行鉴定。

We are investigating the ultrastructure of a hyperthermophilic archaeon, Sulfolobus shibatae, with the hypothesis that the molecular chaperonins in this organism form the basis for a filamentous cytostructure that is a prokaryotic version of the eukaryotic cytoskeleton. The goal is to confirm the presence of filamentous structures previously seen in these organisms and to elucidate their composition; i.e., to establish their relationship to molecular chaperonins. Strategy is to attempt to optimize imaging of cytoplasmic structures using quick-freezing techniques, HR-SEM, and energy-filtering microscopy. Proteins associated with filaments will be identified by immun07gold or fluorescence techniques.