CHAPERONIN (SULFOLOBUS SHIBATAE)

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。该项目将侧重于来自极端嗜热古菌柴田硫化叶菌的第二组伴侣蛋白。S. Shibatae由在所有生长条件下细胞中最丰富的一些蛋白质组成。本项目的目标是利用低温电子显微镜：1）研究伴侣蛋白双环（称为“Rosettasomes”）是否是体内的功能形式，或者是否存在一些高级结构; 2）表征伴侣蛋白的高级结构，以阐明它们在体内的功能，并研究它们如何在纳米技术中使用;和3）可视化热休克前后细胞中的伴侣蛋白结构。除了研究伴侣蛋白在体内的结构和位置，我们将研究伴侣蛋白结构与脂质体和脂质层。这将支持我们在体内寻找这些伴侣蛋白，并将提供重要的结构信息，我们一直在使用的应用程序在纳米技术的伴侣蛋白的高阶结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The project will focus on group II chaperonins from the hyperthermophilic archaeon, Sulfolobus shibatae. The chaperonins in S. shibatae are composed of some of the most abundant proteins in the cells under all growth conditions. The goals of this project are to use cryo-electron microscopy to: 1)investigate if the chaperonin double rings, called "rosettasomes", are the functional form in vivo or if some higher-order structures are present; 2) characterize the higher-order structures of chaperonins to elucidate how they may function in vivo and investigate how they can be used in nanotechnology; and 3) visualize the chaperonin structures in cells before and after heat shock. In addition to investigating the structure and location of chaperonins in vivo, we will investigate chaperonin structures in association with liposomes and lipid layers. This will support our search for these chaperonins in vivo and will provide important structural information about higher-order structures of chaperonins that we have been using for applications in nanotechnology.