CHAPERONIN (SULFOLOBUS SHIBATAE)

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目将重点关注来自超嗜热古菌——柴硫硫化叶菌（Sulfolobus shibatae）的第二类伴侣蛋白。 S. shibatae 中的伴侣蛋白由所有生长条件下细胞中最丰富的一些蛋白质组成。 该项目的目标是利用冷冻电子显微镜： 1）研究伴侣蛋白双环（称为“罗塞塔体”）是否是体内的功能形式，或者是否存在一些高级结构； 2）表征伴侣蛋白的高阶结构，以阐明它们在体内如何发挥作用并研究它们如何在纳米技术中使用； 3）可视化热休克前后细胞中的伴侣蛋白结构。 除了研究体内伴侣蛋白的结构和位置之外，我们还将研究与脂质体和脂质层相关的伴侣蛋白结构。这将支持我们在体内寻找这些伴侣蛋白，并将提供有关我们一直用于纳米技术应用的伴侣蛋白高阶结构的重要结构信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The project will focus on group II chaperonins from the hyperthermophilic archaeon, Sulfolobus shibatae. The chaperonins in S. shibatae are composed of some of the most abundant proteins in the cells under all growth conditions. The goals of this project are to use cryo-electron microscopy to: 1)investigate if the chaperonin double rings, called "rosettasomes", are the functional form in vivo or if some higher-order structures are present; 2) characterize the higher-order structures of chaperonins to elucidate how they may function in vivo and investigate how they can be used in nanotechnology; and 3) visualize the chaperonin structures in cells before and after heat shock. In addition to investigating the structure and location of chaperonins in vivo, we will investigate chaperonin structures in association with liposomes and lipid layers. This will support our search for these chaperonins in vivo and will provide important structural information about higher-order structures of chaperonins that we have been using for applications in nanotechnology.