SHIV CHALLENGE & IN VIVO PASSAGE
艾滋病毒挑战
基本信息
- 批准号:6277563
- 负责人:
- 金额:$ 12.48万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1998
- 资助国家:美国
- 起止时间:1998-05-01 至 1999-04-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Two SHIV challenges have been studied in this project. The first
involved 12 previously immunized animals. Six had been vaccinated
with vaccinia virus expressing HIV-1LAI gp160 and subsequently boosted
with purified subunit gp160 produced by vaccinia virus-infected AGMK
cells. The other six served as mock-vaccinated controls, having been
given vaccinia virus alone and boosted with IFA. Pre-virus challenge
evaluation of the animals revealed serological responses similar to
those achieved in humans immunized with various env preparations. The
macaques were challenged with 25 tissue culture infectious doses
(TCID50) of SHIVIIIB. The six mock-vaccinated animals became
persistently viremic by virus culture and RNA PCR. In contrast, virus
was recovered intermittently from one of the six vaccinated animals
and the RNA PCR revealed virus levels several logs lower in the
vaccinated animals. The animals will continue to be monitored and
will be rechallenged with a more virulent SHIV. In the second
challenge experiment, 22 M. nemestrina divided among 7 experimental
vaccine and control groups also were challenged with SHIVIIIB. The
results supported those in the first challenge experiment, that
vaccinia env prime and env boost afforded the greatest protection
against challenge. Two macaques, one from each control group,
experienced spontaneous CD4 decline. To determine whether virus from
these macaques had become more pathogenic, we gave whole-blood
transfusions to two naive animals. The recipient macaques experienced
a rapid and irreversible CD4 decline. Virus was recovered from the
transfusion recipients and stocks were prepared. To confirm that the
stocks were infectious in vivo, we inoculated two additional macaques
with 1 ml of undiluted virus. These animals also showed CD4
depletion. The stock will be titered in additional macaques to
determine the macaque infectious dose.
本项目研究了两个SHIV挑战。 第一
涉及12只先前免疫的动物。 六人接种了疫苗
用表达HIV-1 LAI gp 160的牛痘病毒,随后加强免疫
用由牛痘病毒感染的AGMK产生的纯化的亚基gp 160
细胞 另外6只作为模拟接种对照,
单独给予牛痘病毒并用IFA加强。 病毒攻毒前
对动物的评价显示血清学反应类似于
这些在用各种env制剂免疫的人中实现。 的
用25个组织培养感染剂量攻击猕猴
(TCID50)。 六只模拟接种疫苗的动物成为
通过病毒培养和RNA PCR检测持续性病毒血症。 相反,病毒
从6只接种动物中的1只中间歇性回收
而RNA PCR显示,
接种疫苗的动物 将继续监测动物,
会被一种毒性更强的SIV病毒再次攻击 在第二
挑战实验,22 M. nemestrina分为7个实验
疫苗组和对照组也用SHIVIIIB攻击。 的
结果支持了第一次挑战实验中的结果,
牛痘env Prime和env加强提供了最大的保护
对抗挑战 两只猕猴,每个对照组一只,
出现自发性CD 4下降。 为了确定病毒是否来自
这些猕猴的致病性越来越强,我们给它们输了全血
输注给两只未处理动物。 受体猕猴经历了
CD 4快速且不可逆的下降。 病毒是从
准备输血受体和储备。 以确认
在动物体内,我们接种了另外两只猕猴
1 ml未稀释的病毒。 这些动物也显示出CD 4
耗尽 该股票将滴定在额外的猕猴,
确定猕猴感染剂量。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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William Randall Morton其他文献
William Randall Morton的其他文献
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{{ truncateString('William Randall Morton', 18)}}的其他基金
IN VIVO EVALUATION OF PRIMATE LENTIVIRUSES II
灵长类慢病毒 II 的体内评估
- 批准号:
7165810 - 财政年份:2005
- 资助金额:
$ 12.48万 - 项目类别:
PRIMATE SUPPLY INFORMATION CLEARINGHOUSE: AIDS
灵长类动物供应信息交换所:艾滋病
- 批准号:
7153978 - 财政年份:2005
- 资助金额:
$ 12.48万 - 项目类别:
EXTRAMURAL RES FACIL IMPROVEMENT PROGRAM PROJ*:EMERGING INFECTIOUS DISEASES
校外资源设施改进计划*:新发传染病
- 批准号:
6973064 - 财政年份:2004
- 资助金额:
$ 12.48万 - 项目类别:
EXTRAMURAL RES FACIL IMPROVEMENT PROGRAM PROJ*: BIODEFENSE
校外资源设施改进计划*:生物防御
- 批准号:
6973063 - 财政年份:2004
- 资助金额:
$ 12.48万 - 项目类别:
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