课题基金 / 基金详情

REDUCE BACKGROUND AUTOFLUORESCENCE IN CELLS: DETECT HIV, PCR REACTIONS

REDUCE BACKGROUND AUTOFLUORESCENCE IN CELLS: DETECT HIV, PCR REACTIONS
减少细胞中的背景自发荧光：检测 HIV、PCR 反应
批准号：
6280926
负责人：
JOHN A STEINKAMP
金额：
$1.02万
依托单位：
UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
项目状态：
已结题
起止时间：
1998-07-01 至 1999-06-30
来源：
https://reporter.nih.gov/project-details/6280926
关键词：
AIDS biomedical equipment development biomedical resource immunology infection inflammation lymphatic system technology /technique virus

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中文摘要
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英文摘要
The autofluorescence of cells that have been subjected to PCR protocols is increased. This elevated autofluorescence interferes with the sensitivity of mRNA measurements. The phase sensitive flow cytometer will be applied to the problem to determine if differences in fluorescence lifetime can be used to reduce the measurement background. The application will be to quantifying HIV mRNA levels in cells with the aim of detecting lower levels than can presently be detected.
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FLOW CYTOMETER FOR QUANTIFYING FLUORESCENCE LIFETIME
IMMUNOFLUORESCENCE PHASE SENSITIVE FLOW CYTOMETRY: CELL CYCLE
REDUCE BACKGROUND AUTOFLUORESCENCE IN CELLS: DETECT HIV, PCR REACTIONS
REDUCE BACKGROUND AUTOFLUORESCENCE IN CELLS: DETECT HIV, PCR REACTIONS