REDUCE BACKGROUND AUTOFLUORESCENCE IN CELLS: DETECT HIV, PCR REACTIONS

减少细胞中的背景自发荧光：检测 HIV、PCR 反应

• 批准号: 6327947
• 负责人: JOHN A STEINKAMP
• 金额: $ 0.57万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6327947
• 关键词: animal tissue; biomedical resource; biotechnology; genetics; technology /technique

The autofluorescence of cells that have been subjected to PCR protocols is increased. This elevated autofluorescence interferes with the sensitivity of mRNA measurements. The phase sensitive flow cytometer will be applied to the problem to determine if differences in fluorescence lifetime can be used to reduce the measurement background. The application will be to quantifying HIV mRNA levels in cells with the aim of detecting lower levels than can presently be detected.

经过聚合酶链式反应的细胞的自体荧光 协议增加。这种升高的自发荧光干扰了 与信使核糖核酸测定的灵敏度相一致。相敏流 将细胞仪应用于该问题以确定是否存在差异 In荧光寿命可以用来减少测量 背景资料。该技术的应用将是量化人类免疫缺陷病毒的基因水平。 细胞，目的是检测比目前更低的水平 检测到。