IMMUNOFLUORESCENCE PHASE SENSITIVE FLOW CYTOMETRY: CELL CYCLE

免疫荧光相敏流式细胞术：细胞周期

• 批准号: 6119991
• 负责人: JOHN A STEINKAMP
• 金额: $ 0.46万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119991
• 关键词: animal tissue; biomedical resource; biotechnology; genetics; technology /technique

The specific aim of this project is to increase the signal-to-noise ratio (S/N) for immunofluorescence flow cytometry detection of intracellular cell cycle regulator molecules by eliminating sources of background noise by phase-sensitive detection methods. At present, p53 cannot be detected in large cells, e.g., fibroblasts and epithelial cell, because of the background noise associated with laser scatter, Raman scatter, nonspecific probe binding, and intrinsic cellular background autofluorescence. We will investigate the use of phase-sensitive flow cytometry to reduce or eliminate these types of interference and thus increase the S/N ratio in low-level measurements. Experiments are currently underway to determine the lifetimes of unstained (fixed) fibroblasts and cells labeled with propidium iodide and proliferating nuclear antigen (PCNA-FITC) and to perform phase-resolved emission measurements of DNA content and immunofluorescence.

这个项目的具体目的是增加