ANALYSIS OF CALCIUM RESPONSES IN NEUTROPHILS BY RAPID MIX FLOW CYTOMETRY

通过快速混合流式细胞术分析中性粒细胞的钙反应

• 批准号: 6280959
• 负责人: JOHN P NOLAN
• 金额: $ 1.91万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6280959
• 关键词: animal tissue; biomedical equipment development; biomedical resource; immunology; inflammation; technology /technique

Calcium transients in neutrophils have been measured with the rapid mix flow cytometer. We have observed that the lag time for the response (2 seconds) is longer than the response time of the instrument (300 msecs). The ability to stimulate the cells uniformly has enabled us to detect cell populations in transition between minimal and maximal calcium levels. This project can be resumed with the new rapid mix device after addition of a second PMT to the rapid mix flow cytometer to make possible simultaneous analysis of ligand-receptor interactions and cell response.

中性粒细胞中的钙瞬变已经用 快速混合流式细胞仪 我们已经观察到， 响应时间（2秒）长于 仪器（300毫秒）。 均匀刺激细胞的能力 使我们能够检测到细胞群在 最低和最高钙水平。 该项目可以恢复， 向快速混合装置中添加第二个PMT后， 混合流式细胞仪可以同时分析 配体-受体相互作用和细胞反应。