ROLE OF OXYGEN IN DEVELOPMENT OF ECHINODERM EMBRYO

氧选择自参考探头正被用于测试 如果在显微镜下氧气可利用性的差异 制剂是胚胎中观察到的抑制作用的原因 发展。这些实验使用了一个经典的模型 发育组织，外胚层卵子和胚胎以及 跟随井上的观察，胚胎的位置，在汇合处 质量，会导致不同步的增长。最初的研究是 旨在将氧通量的变化记录为 未受精卵和胚胎，并观察氧气消耗 随着胚胎的发育而变化。到目前为止，我们已经能够展示 氧流量的显著增加与 受精过程。此外，我们已经能够测量出 这些变化的实时动力学。当实验正在进行时 在早期阶段，该系统已经显示出进一步发展的前景 研究。棘皮动物系统非常容易使用。 从一只动物身上可以获得数百万个配子，而这些 配子在室温的海水中仍能存活数小时。 因此，卵母细胞受精和合子形成是可以启动的。 在调查员方便的时候，确切的时间可以是 注意到了。记录小鼠耗氧量变化的研究 胚胎得到了这项研究的补充。毫无疑问，这将是 将这些发现进行比较是有价值的。海星胚胎 在氧气充足的水中发育，而小鼠胚胎 在低氧的子宫环境中发育。总体而言，这些研究 有助于我们理解新陈代谢的变化 与早期胚胎发育有关。

The oxygen-selective self-referencing probe is being used to test if differences in oxygen availa-bility within a microscopic preparation are responsible for observed inhibition in embryo develop-ment. The experiments are using a classic model of developmental organization, the echninoderm ooyte and embryo and follow observations by Inoue that embryo position, in a confluent mass, causes asynchronous growth. The initial research has been oriented toward documenting the changes in oxygen flux into unfertilized eggs and embryos, and seeing how oxygen consumption changes as the embryo develops. To date we have been able to show that a significant increase in the oxygen influx is associated with the fertilization process. Furthermore, we have been able to measure the kinetics of these changes in real time. While experiments are in the early stages, this system has shown much promise for further research. The echinoderm system is extremely easy to work with. Millions of gametes can be obtained from a single animal and these gametes remain viable for hours in seawater at room temperature. Therefore, oocyte fertilization and zygote formation can be initiated at the convenience of the investigator and the exact time can be noted. The studies documenting changes in oxygen consumption in mouse embryos is complemented by this research. It will undoubtedly be valuable to compare the findings from these. The starfish embryo develops in reasonably well oxygenated waters whereas the mouse embryo develops in the hypoxic uterine environment. Overall these studies will contribute to our understanding of the metabolic changes associated with early embryonic development.