ROLE OF OXYGEN IN DEVELOPMENT OF ECHINODERM EMBRYO

氧气在棘皮动物胚胎发育中的作用

• 批准号: 6568414
• 负责人: D M PORTERFIELD
• 金额: $ 21.13万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6568414
• 关键词: Invertebrata; bioengineering /biomedical engineering; biological products; biomedical equipment development; biomedical resource; growth /development; human tissue; model design /development; reproductive system; technology /technique

The oxygen-selective self-referencing probe is being used to test if differences in oxygen availa-bility within a microscopic preparation are responsible for observed inhibition in embryo develop-ment. The experiments are using a classic model of developmental organization, the echninoderm ooyte and embryo and follow observations by Inoue that embryo position, in a confluent mass, causes asynchronous growth. The initial research has been oriented toward documenting the changes in oxygen flux into unfertilized eggs and embryos, and seeing how oxygen consumption changes as the embryo develops. To date we have been able to show that a significant increase in the oxygen influx is associated with the fertilization process. Furthermore, we have been able to measure the kinetics of these changes in real time. While experiments are in the early stages, this system has shown much promise for further research. The echinoderm system is extremely easy to work with. Millions of gametes can be obtained from a single animal and these gametes remain viable for hours in seawater at room temperature. Therefore, oocyte fertilization and zygote formation can be initiated at the convenience of the investigator and the exact time can be noted. The studies documenting changes in oxygen consumption in mouse embryos is complemented by this research. It will undoubtedly be valuable to compare the findings from these. The starfish embryo develops in reasonably well oxygenated waters whereas the mouse embryo develops in the hypoxic uterine environment. Overall these studies will contribute to our understanding of the metabolic changes associated with early embryonic development.

氧选择性自参比探头用于测试 如果在显微镜下氧气可用性的差异 制剂负责观察到的胚胎抑制 发展。 这些实验使用了一个经典的 棘皮动物卵母细胞和胚胎的发育组织， 根据井上的观察，胚胎的位置，在一个融合的 质量，导致异步增长。 最初的研究是 旨在记录氧气流量的变化， 未受精的卵子和胚胎， 随着胚胎的发育而变化。 到目前为止，我们已经能够证明， 氧气流入量的显著增加与 受精过程。 此外，我们已经能够测量 这些变化的动力学在真实的时间。 当实验在 在早期阶段，这一系统显示出进一步发展的前景。 research. 棘皮动物系统非常容易操作。 从一只动物身上可以获得数百万个配子， 配子在室温下的海水中可存活数小时。 因此，卵母细胞受精和受精卵形成可以启动 在研究者方便的时候，确切的时间可以是 知道了 记录小鼠耗氧量变化的研究 胚胎是这项研究的补充。 无疑将是 比较这些发现是很有价值的。 海星胚胎 在含氧沃茨中发育， 在缺氧的子宫环境中发展。 总体而言， 将有助于我们理解 与早期胚胎发育有关。