PROPIONATE UTILIZATION, GLUCONEOGENESIS, & PYRUVATE RECYCLING IN HUMAN LIVER

丙酸利用、糖异生、

• 批准号: 6121192
• 负责人: ROLF GRUETTER
• 金额: $ 0.82万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 1999-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121192
• 关键词: biomedical resource; carbohydrates; female; human subject; liver; magnetic resonance imaging; male; technology /technique

We have recently performed trial studies with fasted human volunteers given [U-13C]propionate orally. Following ingestion of ~1.5 g [U-13C]propionate, there was a rapid evolution of multiply-enriched plasma glucose species within 2-3 hours, demonstrating rapid and extensive incorporation of [U-13C]propionate units into gluconeogenesis. Carbons 1, 2, 5, and 6 of gluconate showed identical enrichment kinetics, reaching a peak enrichment of about 4.5%, while carbons 3 and 4 reached peak enrichments of about 2.5%. Between 120 and 180 minutes, enrichment of all six glucose carbons remained relatively constant. However, urinary glucuronide labeling, which was monitored up to 6 hours post-ingestion showed a decline in the hexose enrichment setting in at 3-4 hours, indicating depletion of the [U-13C]propionate. No [U-13C]propionate was ever detected in any of the blood plasma samples, indicating its complete removal from the circulation from 0-3 hours post ingestion. For the later time-points, isotopomer analysis of the C2 resonance of glucose provided relative flux values for anaplerosis, pyruvate recycling, and gluconeogenic fluxes of 5.92, 3.55, and 2.37, respectively (relative to a citrate synthase flux of 1.0). These relative flux estimates are consistent with those reported by others using [3-14C]lactate and indicate a high activity of hepatic pyruvate cycling. (Collaborative 9) REPORT PERIOD: (09/01/97-08/31/98)

我们最近对禁食的人类进行了试验研究