CONFORMATION OF CARBOHYDRATE ANTIGENS

SIR提供[U-"C]葡萄糖，而碳水化合物的作用是 在各种重要的生物学现象，研究 其中一些活动受到材料供应的限制。 最近 碳水化合物化学的进展，包括固体 相位合成方法有助于解决这一限制， 制备大量明确定义的 纯材料。 这些方法已经在非洲结出了果实。 制备刘易斯血型成员的碳水化合物。 这些碳水化合物的表达和识别与 与细胞识别、细菌感染和癌症有关， 这些有趣的目标进行构象分析， 他们的生物学角色。 溶液构象的研究 刘易斯x行列式已经使用质子NMR进行， 在天然丰度下的杂波NMR。 在一种情况下， tLewis x寡糖中的一种糖用13C标记， 为构象分析提供了优势。 总的来说 构象分析的实验数据不足以 充分定义结构和分析在很大程度上依赖于 建模 本文提出的制备标记化合物的目的是 为了克服这些局限性， 可用的技术。 新型合成的一个重要方面是， 正在开发的方法是，它们可以有效地结合起来， 同位素标记 这将极大地改善NMR结果， 加强这些spedies的构象分析。 的影响 同位素标记对RNA的研究说明了 这是在研究糖部分时可以获得的优势。

The SIR provided [U-"C]Glucal While the role of carbohydrates is wall recognized in a variety of important biological phenomena, study of them has been restricted by availability of material. Recent advances in carbohydrate chemistry, including development of solid phase synthesis methods have helped address this limitation, offering general protocols for preparing significant quantities of well defined pure material. Such approaches have already bom fruit in the preparation of members of the Lewis blood group carbohydrates. Expression and recognition of these carbohydrates have been associated with cellular recognition, bacterial infection and cancer, making these interesing targets for conformational analysis to understand their biological roles. Investigations of solution conformation of the Lewis x determinant have been undertaken using proton NMR and heteronuclear NMR at natural abundance. In one instance, the C l' of one of the sugars in tLewis x oligosaccharide was labeled with 13C, providing advantages for conformational analysis. In gerneral experimental data for conformational analysis has been insuffcient to fully define the structure and analysis has relied heavily on modeling. The aim of preparing the labeled compounds proposed here is to overcome these limitations byrnaking powerful heteronuclear NMR techniques availabe. An important aspect of the novel synthetic methods being developed is that they can be combined effectively with isotopic labeling. This will dramatically improve the NMR results and enhance the conformational analysis of these spedies. The impact of isotopic labeling on the study of RNA illustrates the kind of advantages that can be gained in studying sugar moieties.