CONFORMATION OF CARBOHYDRATE ANTIGENS

SIR提供葡萄糖，而碳水化合物的作用是 沃尔发现了各种重要的生物现象，研究 其中一些受到材料可获得性的限制。近期 碳水化合物化学的进展，包括固体的开发 相合成方法已经帮助解决了这一限制，提供了 用于制备大量定义明确的 纯净的材料。这样的方法已经在 路易斯血型碳水化合物的制备。 这些碳水化合物的表达和识别与 细胞识别，细菌感染和癌症，使 构象分析要了解的这些有趣的目标 它们的生物学作用。关于溶液构象的研究 Lewis x行列式已使用质子核磁共振和 天然丰度的异核核磁共振。在一个例子中，C L的 用13C标记tLewis x寡糖中的一种糖， 为构象分析提供了优势。在一般情况下 用于构象分析的实验数据还不足以 充分定义结构和分析一直严重依赖于 模特儿。这里提出的制备标记化合物的目的是 通过制造强大的异核核磁共振来克服这些限制 技术是可用的。新的合成技术的一个重要方面 正在开发的方法是它们可以有效地与 同位素标记。这将极大地改善核磁共振结果和 加强对这些物种的构象分析。网络的影响 RNA研究中的同位素标记说明了这种 在研究糖份方面可以获得的优势。

The SIR provided [U-"C]Glucal While the role of carbohydrates is wall recognized in a variety of important biological phenomena, study of them has been restricted by availability of material. Recent advances in carbohydrate chemistry, including development of solid phase synthesis methods have helped address this limitation, offering general protocols for preparing significant quantities of well defined pure material. Such approaches have already bom fruit in the preparation of members of the Lewis blood group carbohydrates. Expression and recognition of these carbohydrates have been associated with cellular recognition, bacterial infection and cancer, making these interesing targets for conformational analysis to understand their biological roles. Investigations of solution conformation of the Lewis x determinant have been undertaken using proton NMR and heteronuclear NMR at natural abundance. In one instance, the C l' of one of the sugars in tLewis x oligosaccharide was labeled with 13C, providing advantages for conformational analysis. In gerneral experimental data for conformational analysis has been insuffcient to fully define the structure and analysis has relied heavily on modeling. The aim of preparing the labeled compounds proposed here is to overcome these limitations byrnaking powerful heteronuclear NMR techniques availabe. An important aspect of the novel synthetic methods being developed is that they can be combined effectively with isotopic labeling. This will dramatically improve the NMR results and enhance the conformational analysis of these spedies. The impact of isotopic labeling on the study of RNA illustrates the kind of advantages that can be gained in studying sugar moieties.