A4: UV RADIATION ON CORNEA & LENS: ANTIOXIDANTS, ENZYME ACTIVITY & GENE EXPRESS

紫外线(UV.)射线，形成 氧自由基，抗氧化酶的失活和 青光眼和老年性白内障的出现 已经成立了。因为平流层臭氧的可能减少 老年人白内障发病率高的原因 人群中，紫外线引起的白内障对公众和环境健康 这意味着什么。我们建议研究紫外线诱导的机理。 角膜和晶状体损伤以及与之相关的机制 用一些低分子量观察其保护作用 抗氧化剂。具体地说，我们建议研究LN的影响 辐射对催化清除自由基氧的酶的影响 物种；与NADPH的产生有关的酶和还原的 谷胱甘肽。这些酶动力学的改变将是 在不同浓度的抗氧化剂存在下测定 如维生素C、维生素E和β-胡萝卜素。此外，我们还 提议看一看 从基因表达水平分离受紫外线影响的基因(S) 在晶状体和角膜组织中进行照射。建议的研究结果 研究可能会使我们更好地了解预防 LN引起的角膜和晶状体混浊，并可能 解决导致预防或延迟的未来研究方法 通过营养抗氧化剂形成白内障的可能性。

The association between ultraviolet (UV.) rays, formation of oxygen free radicals, inactivation of antioxidative enzymes and the appearance of photokeratitis and age-related cataract has been established. Since the possible decrease in the stratosphere's ozone layer as well as the large incidence of cataracts in our elderly population, UV induced cataracts has public and environmental health implications. We propose to study tile mechanisms of UV induced corneal and lens damage as well as those mechanisms associated with the protective effect observed with some low molecular weight antioxidants. Specifically, we propose to study the effect of LN radiation on enzymes that catalyze the removal of radical oxygen species; enzymes related to the generation of NADPH and reduced glutathione. The alteration of these enzymatic kinetics will be determined in the presence of varying concentrations of antioxidants such as vitamin C, vitamin E and beta-carotene. In addition, we also propose to look at the level of gene expression and to isolate gene(s) affected by UV irradiation in lens and cornea tissues. The results of the proposed studies may lead to a better understanding of the prevention of LN-induced opacity of the corneas and the lens and could potentially address future research approaches leading to the prevention or delay of cataract formation through nutritional antioxidants.