CORNEAL EXTRACELLULAR MATRIX METALLOPROTEINASES

细胞外基质金属蛋白酶 (MMP) 活性过高 进入角膜可导致角膜溃疡。 疤痕形成的原因是 角膜溃疡是失明和视力受损的主要原因。 我们担心常用眼药的效果 和紫外线辐射对角膜 MMP 的影响。 我们建议学习四大专业 角膜基质金属蛋白酶：明胶酶 A、明胶酶 B、胶原酶和溶基质素 在器官培养的正常、受伤和患病条件下。 猪 将使用角膜。 眼科药物和紫外线辐射的影响 将评估每个 MMP 的净合成。 的 待测试的药物包括抗菌剂、 抗真菌剂、抗组胺剂、扩瞳剂、抗青光眼、 抗胆碱能和抗炎作用。 这些效果 每种纯化的 MMP 的活性药物也将 研究过。 我们的工作结果可能会带来更好的理解 这些药物和紫外线辐射对结构完整性的影响 角膜。 我们的长期目标是确定 环境因素对角膜结构完整性的影响。

Excessive extracellular matrix metalloproteinases (MMPs) activities in the cornea can lead to cornea ulceration. Cicatrization due to cornea ulceration is a major cause of blindness and impaired vision. We are concerned about the effect of commonly used eye medications and UV radiation on corneal MMPs. We propose to study the four major corneal MMPs: gelatinase A, gelatinase B, collagenase and stromelysin under normal, injured and diseased conditions in organ culture. Pig corneas will be used. The effect of eye medications and UV radiation on the net synthesis of each of these MMPs will be evaluated. The medications to be tested include agents for antibacterial, antifungus, antihistamine, pupil dilator, antiglucoma, anticholinergic and anti-inflammatory. The effect of these medications of the activity of each purified MMPs will also be studied. The results of our work may lead to a better understanding of these medications and UV radiation on the structural integrity of the cornea. Our long term goal is to determine the effect of environmental agents on the structural integrity of the cornea.