CORNEAL EXTRACELLULAR MATRIX METALLOPROTEINASES

细胞外基质金属蛋白酶(MMPs)活性过度 在角膜中会导致角膜溃疡。因以下原因而愈合： 角膜溃疡是导致失明和视力受损的主要原因。 我们对常用眼科药物的效果感到担忧 紫外线照射对角膜基质金属蛋白酶的影响。我们建议研究四大 角膜基质金属蛋白酶：明胶酶A、明胶酶B、胶原酶和基质分解酶 在正常、损伤和疾病条件下进行器官培养。猪 将使用角膜。眼科药物和紫外线辐射的作用 最后，我们将对每一种MMP的合成进行评估。这个 接受检测的药物包括抗菌剂， 抗真菌，抗组胺，瞳孔扩张器，抗青光眼， 抗胆碱能和抗炎。这些因素的影响 每个纯化的MMPs活性的药物也将 学习。我们工作的结果可能会使我们更好地理解 这些药物和紫外线辐射对结构完整性的影响 眼角膜。我们的长期目标是确定 环境因子对角膜结构完整性的影响。

Excessive extracellular matrix metalloproteinases (MMPs) activities in the cornea can lead to cornea ulceration. Cicatrization due to cornea ulceration is a major cause of blindness and impaired vision. We are concerned about the effect of commonly used eye medications and UV radiation on corneal MMPs. We propose to study the four major corneal MMPs: gelatinase A, gelatinase B, collagenase and stromelysin under normal, injured and diseased conditions in organ culture. Pig corneas will be used. The effect of eye medications and UV radiation on the net synthesis of each of these MMPs will be evaluated. The medications to be tested include agents for antibacterial, antifungus, antihistamine, pupil dilator, antiglucoma, anticholinergic and anti-inflammatory. The effect of these medications of the activity of each purified MMPs will also be studied. The results of our work may lead to a better understanding of these medications and UV radiation on the structural integrity of the cornea. Our long term goal is to determine the effect of environmental agents on the structural integrity of the cornea.