Schistosome Calpain as a Vaccine Candidates
Schistosome Calpain as a Vaccine Candidates
批准号:
6413303
负责人:
Afzal A Siddiqui
金额:
$14.8万
依托单位国家:
美国
项目类别:
财政年份:
2001
资助国家:
美国
项目状态:
已结题
起止时间:
2001-08-01 至 2005-07-31
关键词:
antigen antibody reaction biotechnology calpain complementary DNA confocal scanning microscopy cytokine drug screening /evaluation enzyme linked immunosorbent assay gene delivery system immunofluorescence technique intramuscular injections laboratory mouse molecular shape nonhuman therapy evaluation schistosomiasis transfection /expression vector transmission electron microscopy vaccine development vector vaccine
中文摘要
描述(申请人提供):血吸虫病是一种慢性,经常
英文摘要
DESCRIPTION (Provided by Applicant): Schistosomiasis is a chronic, often
debilitating, disease afflicting over 200 million people, an additional 600
million are at risk in 74 countries. Although schistosomicidal agents and other
control measures, including public hygiene and snail control exist, the advent
of an efficacious vaccine still remains the most potentially powerful means for
control of this disease.
Schistosomes interact closely with their host, performing functions such as
nutrient uptake, immune avoidance and attachment. Our goal is to test the
hypothesis that host-exposed, schistosome proteins that undertake such
essential functions can be effective targets of a schistosomiasis vaccine. In
this proposal, we focus on one host interactive protein and utilize the latest
technologies to stimulate and modulate immune responses so as to definitively
test the hypothesis that these proteins will be effective targets of a highly
protective schistosomiasis vaccine.
Our vaccine candidate is calcium activated neutral protease (= calpain,
=sm-p80), which plays a pivotal role in the surface membrane renewal of
schistosomes, a phenomenon which is considered to be a mechanism employed by
hemo-helminths to evade host immunity. The protein has been shown to be exposed
at the host parasite interface and to be naturally immunogenic. Therefore if an
immune response is induced against this protein, it should cause substantial
harm to the worm.
In this application we propose to construct expression vectors containing cDNA
encoding calpain and cytokines for DNA immunization. We will test DNA
immunization protocols using these constructs containing calpain and also
vectors directing co-expression of cytokines to enhance immune recognition of
calpain for its ability to elicit a damaging effect on the survival of
schistosomes. In addition to testing the hypothesis that functionally important
schistosome host interactive proteins make effective vaccine targets, the
results of the proposed experiments should offer additional benefits. Our
studies will provide new data on how these strategies and cytokines succeed in
modifying immune responses to amphitropic proteins. Of greater importance,
these studies should provide new insight into immune mechanisms which can
damage worm parasites and establish a model by which to subsequently study the
effector mechanisms.
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会议论文
Experimental Molecular Vaccines for Schistosomiasis
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批准号:8082076
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项目类别:
-
资助金额:$3.34万
-
财政年份:2010
-
负责人:Afzal A Siddiqui
-
依托单位:
Experimental Molecular Vaccines for Schistosomiasis
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批准号:8109778
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项目类别:
-
资助金额:$30.76万
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财政年份:2010
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负责人:Afzal A Siddiqui
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依托单位:
Experimental Molecular Vaccines for Schistosomiasis
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批准号:7846681
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项目类别:
-
资助金额:$3.34万
-
财政年份:2009
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负责人:Afzal A Siddiqui
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依托单位:
Experimental Molecular Vaccines for Schistosomiasis
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批准号:7743820
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项目类别:
-
资助金额:$32.93万
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财政年份:2008
-
负责人:Afzal A Siddiqui
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依托单位:
Experimental Molecular Vaccines for Schistosomiasis
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批准号:7364845
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项目类别:
-
资助金额:$32.75万
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财政年份:2008
-
负责人:Afzal A Siddiqui
-
依托单位:
Experimental Molecular Vaccines for Schistosomiasis
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批准号:7541807
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项目类别:
-
资助金额:$31.54万
-
财政年份:2008
-
负责人:Afzal A Siddiqui
-
依托单位:
Experimental Molecular Vaccines for Schistosomiasis
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批准号:8207249
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项目类别:
-
资助金额:$66.14万
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财政年份:2008
-
负责人:Afzal A Siddiqui
-
依托单位:
Experimental Molecular Vaccines for Schistosomiasis
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批准号:8150761
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项目类别:
-
资助金额:$0.46万
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财政年份:2008
-
负责人:Afzal A Siddiqui
-
依托单位:
Experimental Molecular Vaccines for Schistosomiasis
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批准号:7996553
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项目类别:
-
资助金额:$60.31万
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财政年份:2008
-
负责人:Afzal A Siddiqui
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依托单位:
Schistosome Calpain as a Vaccine Candidates
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批准号:6570481
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项目类别:
-
资助金额:$4.22万
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财政年份:2001
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负责人:Afzal A Siddiqui
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依托单位:
海外基金