FRAMESHIFT REGULATION OF E COLI RNA POLYMERASE GENES

大肠杆菌 RNA 聚合酶基因的移码调控

基本信息

批准号：
6226480
负责人：
James F. CURRAN
金额：
$ 14.06万
依托单位：
WAKE FOREST UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-06-01 至 2004-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6226480
关键词：
DNA directed RNA polymerase Escherichia coli bacterial genetics bacterial proteins beta galactosidase enzyme activity genetic regulation microorganism culture microorganism growth nucleic acid sequence

项目摘要

DESCRIPTION: Proposed is a study of what may be a novel set of properties for the E. coli RNA polymerase core protein genes (rpoA, B and C genes). These genes are known to be growth rate regulated, and that regulation occurs by several transcriptional and translational mechanisms, but the detailed mechanisms and their coordination are largely obscure. Our sequence analyses show that the 5' ends of these genes contain UUU-Ynn dicodons, which are known to be highly frameshift-prone, and which are strongly avoided in other highly expressed genes. That these UUU-Ynn sequences occur near the 5' ends of all three rpo genes, and that this pattern is shared by diverse bacteria, suggests that these sites may have undiscovered, important functions. We will determine whether these sequences are frameshift-prone and whether frameshifting can contribute to regulation of these genes. The rpoB gene may be especially interesting because it encodes a potential frameshift-polypeptide that would be large enough to have function. This feature is also conserved among diverse bacteria. We will determine whether expression of this polypeptide has any global effect on cell physiology, and whether it specifically affects rpo gene expression. These studies may uncover interesting phenomena in the regulation and expression of the physiologically important rpo genes. This work will also train undergraduates interested in biomedical careers.

描述：提出的研究是对可能是一套新型特性的研究大肠杆菌RNA聚合酶核心蛋白基因（RPOA，B和C基因）。这些已知基因是调节生长速率的，并且调节是由几种转录和翻译机制，但详细机制及其协调在很大程度上是晦涩的。我们的序列分析表明这些基因的5端包含uuu-ynn dicodon，这是已知的高度远程易移，并且在其他高度避免表达的基因。这些uuu-ynn序列发生在所有的5'端附近三个RPO基因，这种模式由多种细菌共享，表明这些站点可能没有发现的重要功能。我们将确定这些序列是否易转移，以及是否可以有助于调节这些基因。 RPOB基因尤其是有趣，因为它编码了可能是足够大以具有功能。此功能在不同的细菌。我们将确定该多肽的表达是否具有任何全球对细胞生理的影响及其是否特别影响RPO基因表达。这些研究可能会发现调节中的有趣现象和生理上重要的RPO基因的表达。这项工作也将培训对生物医学职业感兴趣的大学生。